Lv Jing, Liu Guangyu, Gao Huimin, He Yutao, Tang Xiaorong, Wang Ziqi, Sun Kaikai, Bayazitova Kulbaram, Jiang Zhongliang
College of Animal Science and Technology, Key Laboratory of Animal Genetic, Breeding and Reproduction in Shaanxi Province, Northwest A&F University, Yangling, Shaanxi 712100, China.
Department of Food Security, Agrotechnological Faculty, M. Kozybayev North Kazakhstan University, Petropavlovsk, 150000, Kazakhstan.
Int J Biol Macromol. 2024 Dec;282(Pt 2):136718. doi: 10.1016/j.ijbiomac.2024.136718. Epub 2024 Oct 22.
Trehalose, as a food and feed additive, can regulate physiological and pathological processes by activating autophagy, yet the molecular mechanism of trehalose dominated a rise in autophagy has not been elucidated. This study investigated the mechanisms of trehalose-induced autophagy in porcine granulosa cells (PGCs). Trehalose was internalized into PGCs by endocytosis and caused a temporary change of the lysosome pH, ultimately inducing autophagy. Furthermore, miRNA-seq analysis of PGCs indicated that trehalose upregulated the expression of miR-221-5p_R-4, which can target nuclear receptor binding factor 2 (NRBF2) to induce autophagy of PGCs. In summary, this study reveals that miR-221-5p_R-4 targeting NRBF2 was involved in the autophagy of PGCs challenged by trehalose, which was taken up by PGCs through endocytosis.
海藻糖作为一种食品和饲料添加剂，可通过激活自噬来调节生理和病理过程，然而海藻糖主导自噬增加的分子机制尚未阐明。本研究探讨了海藻糖诱导猪颗粒细胞（PGCs）自噬的机制。海藻糖通过内吞作用进入PGCs，并导致溶酶体pH值的暂时变化，最终诱导自噬。此外，对PGCs的miRNA测序分析表明，海藻糖上调了miR-221-5p_R-4的表达，其可靶向核受体结合因子2（NRBF2）以诱导PGCs自噬。总之，本研究揭示了靶向NRBF2的miR-221-5p_R-4参与了经内吞作用被PGCs摄取的海藻糖所诱导的PGCs自噬。
