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兔横纹肌型尿道外括约肌的纤维类型:肌球蛋白的电泳分析

The fiber type of the rabbits' striated external urethral sphincter: electrophoretic analysis of myosin.

作者信息

Tokunaka S, Murakami U, Okamura K, Miyata M, Yachiku S

出版信息

J Urol. 1986 Feb;135(2):427-30. doi: 10.1016/s0022-5347(17)45658-8.

DOI:10.1016/s0022-5347(17)45658-8
PMID:3944884
Abstract

The fiber type of the rabbits' external urethral sphincter was investigated with O'Farrell's 2-dimensional electrophoresis of constituent proteins of glycerinated muscle, peptide mapping of myosin heavy chains and non-denaturing pyrophosphate gel electrophoresis of myosin. It was determined as fast type by all three methods. The relative proportions of muscle fibers which were estimated from myosin light chain contents were 88 per cent fast type and 12 per cent slow type. On the basis of the results presented, it was shown to be fast twitch muscle, but not identical to the psoas muscle.

摘要

采用奥法雷尔甘油化肌肉组成蛋白二维电泳、肌球蛋白重链肽图谱分析以及肌球蛋白非变性焦磷酸凝胶电泳法,对兔尿道外括约肌的纤维类型进行了研究。通过这三种方法均确定其为快肌纤维类型。根据肌球蛋白轻链含量估算的肌纤维相对比例为:快肌纤维占88%,慢肌纤维占12%。根据所呈现的结果表明,它是快肌,但与腰大肌不同。

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The fiber type of the rabbits' striated external urethral sphincter: electrophoretic analysis of myosin.兔横纹肌型尿道外括约肌的纤维类型:肌球蛋白的电泳分析
J Urol. 1986 Feb;135(2):427-30. doi: 10.1016/s0022-5347(17)45658-8.
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