人尿道外括约肌中快慢肌球蛋白同工酶的共存。初步报告。

Coexistence of fast and slow myosin isozymes in human external urethral sphincter. A preliminary report.

作者信息

Tokunaka S, Murakami U, Fujii H, Okamura K, Miyata M, Hashimoto H, Yachiku S

出版信息

J Urol. 1987 Sep;138(3):659-62. doi: 10.1016/s0022-5347(17)43293-9.

Abstract

Human external urethral sphincter muscle was isolated for biochemical research. Myosin samples were prepared and pyrophosphate gel electrophoresis and two-dimensional electrophoresis were performed. Assignment in pyrophosphate gel of myosin isozymes of human external urethral sphincter was carried out by making comparisons with those from human rectus abdominis muscle. Myosin light chains in human external urethral sphincter were identified using two-dimensional electrophoresis. Human external urethral sphincter, similar to other voluntary muscles, is composed of fast and slow twitch muscle fibers, since fast and slow myosin components were shown in both pyrophosphate gel and two-dimensional electrophoretograms.

摘要

分离出人类尿道外括约肌用于生化研究。制备肌球蛋白样本并进行焦磷酸凝胶电泳和二维电泳。通过与人类腹直肌的肌球蛋白同工酶在焦磷酸凝胶中进行比较，对人类尿道外括约肌的肌球蛋白同工酶进行分类。利用二维电泳鉴定人类尿道外括约肌中的肌球蛋白轻链。与其他随意肌相似，人类尿道外括约肌由快肌纤维和慢肌纤维组成，因为在焦磷酸凝胶电泳图和二维电泳图中均显示出快肌球蛋白和慢肌球蛋白成分。