中国江苏分离的水痘-带状疱疹病毒的基因型。

Genotype of Varicella-zoster virus isolated in Jiangsu, China.

机构信息

Department of General Surgery, Northern Jiangsu People's Hospital, Yangzhou, Jiangsu Province, 225001, China.

Department of Expanded Programme on Immunization, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, Jiangsu Province, 210009, China.

出版信息

Virol J. 2024 Oct 24;21(1):264. doi: 10.1186/s12985-024-02543-3.

DOI:10.1186/s12985-024-02543-3

PMID:39449007

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11515349/

Abstract

OBJECTIVE

To analyze the genotypes of VZV in Jiangsu province to identify vaccine strains and wild strains, providing a molecular biological background for the effective prevention and control of varicella.

METHOD

Stratified sampling was used to collect herpes fluid or throat swab from patients diagnosed with varicella. ORF22 was carried out, and the restriction enzyme site of ORF38, ORF54 and ORF62 were detected.

RESULTS

All 207 virus strains were Clade 2 type by sequencing the PCR products of ORF22. The sequencing results showed that five SNP sites changed compared to the Dumas reference strain(Clade 1). From A to G at 37,902, from T to c at 38,055, from A to C at 38,081, and from G to A at 38,177, from G to A at 39,394. The prevalent VZV genotypes in Jiangsu is consistent with the P-Oka. The restriction enzyme site analysis of PCR amplification products from ORF38 (PstI), ORF54 (BglI), ORF62 (SmaI) showed that all 207 virus strains were wild-type. There were two different types of the wild strains, and 183 strains (88.4%) were PstI (+), BglI (+), SmaI (-). The wild strains between different regions showed no significant differences (χ = 0.05, P = 0.982).

CONCLUSIONS

The prevalent VZV genotypes are Clade 2 and the prevalent virus strains are wild strains in Jiangsu Province, the primary wild strain observed is mainly PstI (+), BglI (+), SmaI (-).

摘要

目的

分析江苏省 VZV 的基因型，鉴定疫苗株和野毒株，为水痘的有效防控提供分子生物学背景。

方法

采用分层抽样方法采集水痘患者的疱疹液或咽拭子。进行 ORF22 扩增，并检测 ORF38、ORF54 和 ORF62 的酶切位点。

结果

通过 ORF22 的 PCR 产物测序，所有 207 株病毒均为 Clade 2 型。测序结果与 Dumas 参考株（Clade 1）相比，有 5 个 SNP 位点发生了变化。在 37902 位从 A 突变为 G，在 38055 位从 T 突变为 c，在 38081 位从 A 突变为 C，在 38177 位从 G 突变为 A，在 39394 位从 G 突变为 A。江苏流行的 VZV 基因型与 P-Oka 一致。ORF38（PstI）、ORF54（BglI）、ORF62（SmaI）的 PCR 扩增产物酶切分析显示，所有 207 株病毒均为野生型。有两种不同类型的野生株，183 株（88.4%）为 PstI（+）、BglI（+）、SmaI（-）。不同地区的野生株无显著差异（χ=0.05，P=0.982）。