Department of Obstetrics and Gynecology, The People's Hospital of Guangxi Zhuang Autonomous Region, Guangxi Academy of Medical Sciences, Nanning, China.
Department of Obstetrics and Gynecology, People's Hospital of Guangxi Zhuang Autonomous Region, Guangxi Academy of Medical Sciences, No. 6, Taoyuan Road, Qingxiu District, Nanning, China.
BMC Pregnancy Childbirth. 2024 Oct 24;24(1):696. doi: 10.1186/s12884-024-06907-z.
This research analyzes the potential of long non-coding RNAs (lncRNAs) as markers in determining the necessity of antiviral treatment in pregnant women by examining alterations in the expression profile of serum lncRNAs in pregnant women with elevated hepatitis B viral load (HBVL) under antiviral and non-antiviral treatment regimens between the second trimester and delivery.
Serum was obtained from 6 s-trimester pregnant women with high HBVL and no intrauterine infection. Then, 3 of these women were randomly selected for antiviral treatment, with the remaining 3 women undergoing non-antiviral treatment as control. Serum samples were again collected from these 6 women before delivery. The expression profile of lncRNAs was analyzed with microarray technology, followed by Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses. The axes of hub lncRNA-miRNA-mRNA were identified based on the competing endogenous RNA (ceRNA) network.
The expression profile of serum lncRNAs in pregnant women with high HBVL changed significantly from the second trimester of pregnancy until delivery under antiviral or non-antiviral treatment. The Venn diagram was utilized to screen out the jointly up-regulated and down-regulated lncRNAs in the serum of pregnant women under antiviral and non-antiviral treatment before delivery. Additionally, the KEGG pathway enrichment analysis results showed that lncRNAs might mediate the Hippo pathway in HBV infection. Based on the ceRNA network, 3 hub lncRNAs (CATG00000076041.1, LINC01310, and G014655) were found to potentially regulate the key gene TP73 in the Hippo pathway.
In this study, we retrieved co-differentially expressed lncRNAs in pregnant women with high HBVL under antiviral or non-antiviral treatment, which may be used as markers for evaluating whether pregnant women with high HBVL may be free of antiviral treatment. This study may provide a basis for preventing potential adverse effects of antiviral treatment on maternal and fetal health.
本研究通过分析在抗病毒和非抗病毒治疗方案下，妊娠中期至分娩时高乙型肝炎病毒载量（HBVL）孕妇血清长链非编码 RNA（lncRNA）表达谱的变化，探讨 lncRNA 作为判断孕妇是否需要抗病毒治疗的标志物的潜力。
采集 6 例高 HBVL 且无宫内感染的妊娠晚期孕妇血清。然后，随机选择其中 3 例进行抗病毒治疗，其余 3 例作为对照进行非抗病毒治疗。再次采集这 6 例孕妇分娩前的血清样本。采用微阵列技术分析 lncRNA 的表达谱，然后进行京都基因与基因组百科全书（KEGG）通路富集分析。基于竞争内源性 RNA（ceRNA）网络，确定关键 lncRNA-miRNA-mRNA 轴。
抗病毒或非抗病毒治疗下高 HBVL 孕妇的血清 lncRNA 表达谱从妊娠中期到分娩时均发生显著变化。利用 Venn 图筛选出抗病毒和非抗病毒治疗前孕妇血清中共同上调和下调的 lncRNA。此外，KEGG 通路富集分析结果表明，lncRNA 可能在乙型肝炎病毒感染中介导 Hippo 通路。基于 ceRNA 网络，发现 3 个关键 lncRNA（CATG00000076041.1、LINC01310 和 G014655）可能调节 Hippo 通路中的关键基因 TP73。
本研究在抗病毒和非抗病毒治疗下高 HBVL 孕妇中检索出差异共表达的 lncRNA，这些 lncRNA 可能可作为评估高 HBVL 孕妇是否可免于抗病毒治疗的标志物。本研究可为预防抗病毒治疗对母婴健康的潜在不良影响提供依据。
