Rockstroh Caroline, Hintz Katja, Kannenberg Judith, Jassoy Christian
Institute for Medical Microbiology and Virology, University Hospital and Medical Faculty, University of Leipzig, 04103 Leipzig, Germany.
Antibodies (Basel). 2024 Oct 3;13(4):81. doi: 10.3390/antib13040081.
BACKGROUND/OBJECTIVES: The body's immune response to infections and vaccination leads to the formation of memory B cells (MBCs), which protect against future infections. MBCs circulate in the blood, and the strength of the MBC response is measured with different tests. In this study, tests to measure the MBC response were compared.
An MBC enzyme-linked immunospot assay (MBC-ELISpot), which counts the antibody-releasing cells (MASCs) that arise from memory B cells in vitro, and two versions of an MBC enzyme-linked immunosorbent assay (MBC-ELISA), which measures the concentration of antibodies released by the MASCs, were compared. The lower measurement limit of MBC-ELISpot and ELISA was determined, and it was investigated how the measurement results of individual samples and in a sample of test persons correlate.
Both methods had similar lower limits of detection, and the antibody concentration correlated strongly with the number of MASCs in individual samples. The antibody concentrations measured on a sample correlated with Pearson correlation coefficients of R = 0.83-0.87 with the number of MASCs, and the proportion of antigen-specific antibodies in total IgG correlated with R = 0.74-0.82 with the proportion of antigen-specific MASCs in all antibody-secreting cells.
Since the measurement sensitivity of MBC-ELISA and MBC-ELISpot is similar and the measurement results correlate strongly in a random sample, the tests are interchangeable. The MBC-ELISA has an advantage over the ELISpot in that the antibody measurements can be divided up over time, repeated, and extended. This creates new possibilities for measuring the MBC response.
背景/目的：机体对感染和疫苗接种的免疫反应会导致记忆B细胞（MBC）的形成，这些细胞可预防未来的感染。MBC在血液中循环，可通过不同测试来衡量MBC反应的强度。在本研究中，对衡量MBC反应的测试进行了比较。
比较了一种体外计数源自记忆B细胞的抗体释放细胞（MASC）的MBC酶联免疫斑点试验（MBC-ELISpot），以及两种测量MASC释放抗体浓度的MBC酶联免疫吸附试验（MBC-ELISA）。确定了MBC-ELISpot和ELISA的较低测量限，并研究了个体样本以及测试人群样本的测量结果之间的相关性。
两种方法的检测下限相似，且个体样本中的抗体浓度与MASC数量密切相关。样本中测得的抗体浓度与MASC数量的Pearson相关系数R = 0.83 - 0.87，总IgG中抗原特异性抗体的比例与所有抗体分泌细胞中抗原特异性MASC的比例的相关系数R = 0.74 - 0.82。
由于MBC-ELISA和MBC-ELISpot的测量灵敏度相似，且在随机样本中测量结果高度相关，因此这两种测试可相互替换。MBC-ELISA相对于ELISpot的优势在于抗体测量可随时间进行划分、重复并扩展。这为测量MBC反应创造了新的可能性。
