Santovito Alfredo, Lambertini Mattia, Nota Alessandro
Department of Life Sciences and Systems Biology, University of Turin, Via Accademia Albertina 13, 10123 Torino, Italy.
Department of Chemistry, University of Turin, Via P. Giuria 7, 10125 Torino, Italy.
J Xenobiot. 2024 Oct 8;14(4):1415-1431. doi: 10.3390/jox14040079.
The ubiquitous presence of plastics represents a global threat for all ecosystems and human health. In this study, we evaluated, in vitro and in vivo, the genotoxic potential of different concentrations of polystyrene microplastics (PS-MPs) and their possible synergistic interactions with bisphenol-A (BPA). For the in vitro and the in vivo assays, we used human lymphocytes and hemocytes from , respectively. The genomic damage was evaluated by the micronucleus assay, and differences in eggs laid and growth of were also evaluated. In human lymphocytes, PS-MPs alone at the concentration of 200 μg/mL and in association with BPA 0.100 µg/mL significantly increased the frequencies of micronuclei and nuclear buds, indicating a possible in vitro genotoxic additive action of these two compounds. Vice versa, PS-MPs did not result in genotoxicity in hemocytes. Our results indicated that PS-MPs have genotoxic properties only in vitro and at a concentration of 200 µg/mL; moreover, this compound could intensify the genomic damage when tested with BPA, indicating possible cumulative effects. Finally, PS significantly reduced the growth and the number of laid eggs in
塑料的广泛存在对所有生态系统和人类健康构成了全球性威胁。在本研究中，我们在体外和体内评估了不同浓度的聚苯乙烯微塑料（PS-MPs）的遗传毒性潜力及其与双酚A（BPA）可能的协同相互作用。对于体外和体内试验，我们分别使用了人类淋巴细胞和血细胞。通过微核试验评估基因组损伤，同时也评估了产卵量和生长情况的差异。在人类淋巴细胞中，浓度为200μg/mL的PS-MPs单独存在以及与0.100μg/mL的BPA联合存在时，显著增加了微核和核芽的频率，表明这两种化合物在体外可能具有遗传毒性相加作用。反之，PS-MPs在血细胞中未产生遗传毒性。我们的结果表明，PS-MPs仅在体外且浓度为200μg/mL时具有遗传毒性；此外，当与BPA一起测试时，该化合物可能会加剧基因组损伤，表明可能存在累积效应。最后，PS显著降低了……的生长和产卵数量
