Sartore Giovanni, Piarulli Francesco, Ragazzi Eugenio, Mallia Alice, Ghilardi Stefania, Carollo Massimo, Lapolla Annunziata, Banfi Cristina
Department of Medicine-DIMED, University of Padova, 35122 Padova, Italy.
Studium Patavinum, University of Padova, 35122 Padova, Italy.
Proteomes. 2024 Oct 11;12(4):29. doi: 10.3390/proteomes12040029.
: Diabetes, particularly type 2 diabetes (T2D), is linked with an increased risk of developing coronary heart disease (CHD). The present study aimed to evaluate potential circulating biomarkers of CHD by adopting a targeted proteomic approach based on proximity extension assays (PEA). : The study was based on 30 patients with both T2D and CHD (group DC), 30 patients with T2D without CHD (group DN) and 29 patients without diabetes but with a diagnosis of CHD (group NC). Plasma samples were analyzed using PEA, with an Olink Target 96 cardiometabolic panel expressed as normalized protein expression (NPX) units. : Lysosomal Pro-X carboxypeptidase (PRCP), Liver carboxylesterase 1 (CES1), Complement C2 (C2), and Intercellular adhesion molecule 3 (ICAM3) were lower in the DC and NC groups compared with the DN groups. Lithostathine-1-alpha (REG1A) and Immunoglobulin lambda constant 2 (IGLC2) were found higher in the DC group compared to DN and NC groups. ROC analysis suggested a significant ability of the six proteins to distinguish among the three groups (whole model test < 0.0001, AUC 0.83-0.88), with a satisfactory discriminating performance in terms of sensitivity (77-90%) and specificity (70-90%). A possible role of IGLC2, PRCP, and REG1A in indicating kidney impairment was found, with a sensitivity of 92% and specificity of 83%. : The identified panel of six plasma proteins, using a targeted proteomic approach, provided evidence that these parameters could be considered in the chronic evolution of T2D and its complications.
糖尿病，尤其是2型糖尿病（T2D），与患冠心病（CHD）风险增加有关。本研究旨在通过采用基于邻位延伸分析（PEA）的靶向蛋白质组学方法评估冠心病潜在的循环生物标志物。
该研究纳入了30例患有T2D和CHD的患者（DC组）、30例患有T2D但无CHD的患者（DN组）以及29例无糖尿病但诊断为CHD的患者（NC组）。使用PEA分析血浆样本，采用Olink Target 96心脏代谢检测板，结果以标准化蛋白表达（NPX）单位表示。
与DN组相比，DC组和NC组的溶酶体脯氨酸羧肽酶（PRCP）、肝脏羧酸酯酶1（CES1）、补体C2（C2）和细胞间黏附分子3（ICAM3）水平较低。与DN组和NC组相比，DC组的岩藻糖结合蛋白1-α（REG1A）和免疫球蛋白λ恒定区2（IGLC2）水平较高。ROC分析表明，这六种蛋白质具有显著区分三组的能力（整体模型检验<0.0001，AUC为0.83 - 0.88），在敏感性（77 - 90%）和特异性（70 - 90%）方面具有令人满意的鉴别性能。发现IGLC2、PRCP和REG1A在提示肾脏损害方面可能发挥作用，敏感性为92%，特异性为83%。
通过靶向蛋白质组学方法鉴定出的六种血浆蛋白组成的检测板，为在T2D及其并发症的慢性进展中考虑这些参数提供了证据。
