Safi Ihab N, Hussein Basima Mohammed Ali, Al-Khafaji Aseel Mohammed, Fatalla Abdalbseet A, Al-Shammari Ahmed M
Department of Prosthodontics, College of Dentistry, University of Baghdad, Baghdad, Iraq.
Department of Biomedical Applications, Institute of Laser for Postgraduate Studies, University of Baghdad, Baghdad, Iraq.
Int J Dent. 2024 Oct 17;2024:2571976. doi: 10.1155/2024/2571976. eCollection 2024.
Stem cells are introduced to regenerate some living tissue to restore function and longevity. The study aims to isolate in vitro human periodontal ligament stem cells (hPDLSCs) and investigate their proliferation rate on plasma-treated aligned and random polycaprolactone (PCL) nanofibrous scaffolds made via an electrospinning technique to attempt periodontal-like tissue in dental implants. hPDLSCs were isolated from extracted human premolars and cultured on plasma-treated or untreated PCL-aligned and random scaffolds to enhance adhesion of periodontal ligament (PDL) cells as well as interaction and proliferation. Cell morphology, adhesion, and proliferation rate were evaluated using field emission scanning electron microscopy (FESEM) and the methyl tetrazolium (MTT) assay. The wettability of PCL scaffolds was tested using a goniometer. The hydrophilicity of plasma-treated scaffolds was significantly increased ( ≤ 0.05) in both aligned and random nanofibers compared to the nontreated nanofibrous scaffold. Cells arranged in different directions on the random nanofiber scaffold, while for aligned scaffold nanofibers, the cells were arranged in a pattern that followed the direction of the aligned electrospun nanofibres. The rate of hPDLSC proliferation on an aligned PCL nanofiber scaffold was significantly higher than on a random PCL nanofibrous scaffold with a continuous, well-arranged monolayer of cells, as shown in FESEM. The aligned PCL nanofiber scaffold is superior to random PCL when used as an artificial scaffold for hPDLSC regeneration in PDL tissue engineering applications.
引入干细胞以再生一些活组织，从而恢复功能和延长寿命。该研究旨在体外分离人牙周膜干细胞（hPDLSCs），并研究其在通过静电纺丝技术制备的经等离子体处理的定向和随机聚己内酯（PCL）纳米纤维支架上的增殖率，以尝试在牙种植体中构建牙周样组织。从拔除的人前磨牙中分离出hPDLSCs，并将其培养在经等离子体处理或未处理的PCL定向和随机支架上，以增强牙周膜（PDL）细胞的黏附以及相互作用和增殖。使用场发射扫描电子显微镜（FESEM）和甲基四氮唑（MTT）法评估细胞形态、黏附及增殖率。使用测角仪测试PCL支架的润湿性。与未处理的纳米纤维支架相比，经等离子体处理的支架在定向和随机纳米纤维中的亲水性均显著增加（≤0.05）。细胞在随机纳米纤维支架上沿不同方向排列，而对于定向支架纳米纤维，细胞则沿定向电纺纳米纤维的方向排列。如FESEM所示，hPDLSCs在定向PCL纳米纤维支架上的增殖率显著高于在随机PCL纳米纤维支架上，且细胞形成连续、排列良好的单层。在PDL组织工程应用中，当用作hPDLSC再生的人工支架时，定向PCL纳米纤维支架优于随机PCL支架。
