Zhuikov Vsevolod, Zhuikova Yulia
Research Center of Biotechnology of the Russian Academy of Sciences, 33, bld. 2 Leninsky Ave., 119071 Moscow, Russia.
Gels. 2024 Oct 17;10(10):664. doi: 10.3390/gels10100664.
Poly(3-hydroxybutyrate) (PHB) is a microbially derived polyhydroxyalkanoate that is widely used in biomedical applications. In this study, we investigated the use of acetic acid (aa) as an alternative environmentally friendly solvent for the preparation of gels from PHB (PHB aa) and compared their characteristics with PHB products dissolved in chloroform (PHB chl) using such methods as DSC, FTIR, SEM, rheometry, biodegradation, and cytocompatibility assay. A slight decrease in the degree of the crystallinity of the PHB from 61% to 50.8% was found when the acetic acid was used. This resulted in a greater mass loss for the PHB aa (11%) during enzymatic degradation over 180 days. Gels prepared from PHB in the different solvents showed differences in the microstructure and porosity of the samples, which affected their viscoelastic properties. The storage modulus (G') for the PHB aa gels was higher by 35% compared to that for the PHB chl, and Young's modulus in compression was 101.5 and 41.3 kPa for the PHB aa and PHB chl, respectively. The porosity of the PHB aa was 97.7%, which was 5.2% higher than that for the PHB chl. The presence of low molecular weight polymers in the PHB aa had an effect on mesenchymal stem cells' viability, expressed as a threefold increase in the number of attached cells after 7 days of incubation compared to the PHB chl. Thus, the proposed method of PHB-based materials' preparation is a promising, more environmentally friendly analog of the extensively used method of preparation from chloroform.
聚（3-羟基丁酸酯）（PHB）是一种微生物来源的聚羟基脂肪酸酯，广泛应用于生物医学领域。在本研究中，我们研究了使用乙酸（aa）作为一种替代的环境友好型溶剂来制备PHB凝胶（PHB aa），并使用差示扫描量热法（DSC）、傅里叶变换红外光谱法（FTIR）、扫描电子显微镜法（SEM）、流变学、生物降解和细胞相容性测定等方法，将其特性与溶解在氯仿中的PHB产品（PHB chl）进行比较。当使用乙酸时，发现PHB的结晶度略有下降，从61%降至50.8%。这导致PHB aa在180天的酶促降解过程中质量损失更大（11%）。在不同溶剂中由PHB制备的凝胶在样品的微观结构和孔隙率方面存在差异，这影响了它们的粘弹性。与PHB chl相比，PHB aa凝胶的储能模量（G'）高35%，PHB aa和PHB chl在压缩时的杨氏模量分别为101.5和41.3 kPa。PHB aa的孔隙率为97.7%，比PHB chl高5.2%。PHB aa中低分子量聚合物的存在对间充质干细胞的活力有影响，与PHB chl相比，孵育7天后附着细胞数量增加了三倍。因此，所提出的基于PHB的材料制备方法是一种有前景的、更环保的类似物，可替代广泛使用的氯仿制备方法。
