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微重力作为研究胸膜间皮细胞癌症诱导的工具。

Microgravity as a Tool to Investigate Cancer Induction in Pleura Mesothelial Cells.

作者信息

Bonetto Valentina, Pagano Corinna Anais, Sabbatini Maurizio, Magnelli Valeria, Donadelli Massimo, Marengo Emilio, Masini Maria Angela

机构信息

Department of Science and Innovation Technology (DISIT), Università del Piemonte Orientale, 15121 Alessandria, Italy.

Department of Neurosciences, Biomedicine and Movement Sciences (DNBM), University of Verona, 37124 Verona, Italy.

出版信息

Curr Issues Mol Biol. 2024 Sep 27;46(10):10896-10912. doi: 10.3390/cimb46100647.

DOI:10.3390/cimb46100647
PMID:39451527
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11506709/
Abstract

The present work shows that the exposure of mesothelial cells to simulated microgravity changes their cytoskeleton and adhesion proteins, leading to a cell switch from normal towards tumoral cells. Immunohistochemical and molecular data were obtained from both MeT-5A exposed to simulated microgravity and BR95 mesothelioma cell lines. Simulated microgravity was found to affect the expression of actin, vinculin, and connexin-43, altering their quantitative and spatial distribution pattern inside the cell. The analysis of the tumoral markers p27, CD44, Fibulin-3, and NANOG and the expression of genes related to cancer transformation such as NANOG, CDH-1, and Zeb-1 showed that the simulated microgravity environment led to expression patterns in MeT-5A cells similar to those observed in BR95 cells. The alteration in both quantitative expression and structural organization of the cytoskeleton and adhesion/communication proteins can thus be considered a pivotal mechanism involved in the cellular shift towards tumoral progression.

摘要

目前的研究表明，间皮细胞暴露于模拟微重力环境会改变其细胞骨架和粘附蛋白，导致细胞从正常细胞转变为肿瘤细胞。免疫组织化学和分子数据来自暴露于模拟微重力环境的MeT-5A细胞系和BR95间皮瘤细胞系。研究发现，模拟微重力会影响肌动蛋白、纽蛋白和连接蛋白43的表达，改变它们在细胞内的定量和空间分布模式。对肿瘤标志物p27、CD44、纤连蛋白3和NANOG以及与癌症转化相关基因（如NANOG、CDH-1和Zeb-1）表达的分析表明，模拟微重力环境导致MeT-5A细胞中的表达模式与BR95细胞中观察到的相似。因此，细胞骨架以及粘附/通讯蛋白在定量表达和结构组织上的改变可被视为参与细胞向肿瘤进展转变的关键机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a734/11506709/84173ab02932/cimb-46-00647-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a734/11506709/e68524cac240/cimb-46-00647-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a734/11506709/8907553987d5/cimb-46-00647-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a734/11506709/0c5d25471119/cimb-46-00647-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a734/11506709/d1da04f17c31/cimb-46-00647-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a734/11506709/14d717ab10b3/cimb-46-00647-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a734/11506709/84173ab02932/cimb-46-00647-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a734/11506709/e68524cac240/cimb-46-00647-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a734/11506709/8907553987d5/cimb-46-00647-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a734/11506709/0c5d25471119/cimb-46-00647-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a734/11506709/d1da04f17c31/cimb-46-00647-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a734/11506709/14d717ab10b3/cimb-46-00647-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a734/11506709/84173ab02932/cimb-46-00647-g006.jpg

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本文引用的文献

1
The relationship between cancer and biomechanics.癌症与生物力学之间的关系。
Front Oncol. 2023 Oct 12;13:1273154. doi: 10.3389/fonc.2023.1273154. eCollection 2023.
2
Chronic Inflammation, Oxidative Stress and Metabolic Plasticity: Three Players Driving the Pro-Tumorigenic Microenvironment in Malignant Mesothelioma.慢性炎症、氧化应激与代谢可塑性：共同驱动间皮瘤致瘤微环境的三要素。
Cells. 2023 Aug 11;12(16):2048. doi: 10.3390/cells12162048.
3
Cytomembrane Trafficking Pathways of Connexin 26, 30, and 43.间隙连接蛋白 26、30 和 43 的细胞内膜转运途径。
Int J Mol Sci. 2023 Jun 19;24(12):10349. doi: 10.3390/ijms241210349.
4
The evolving tumor microenvironment: From cancer initiation to metastatic outgrowth.不断演变的肿瘤微环境：从癌症起始到转移灶生长
Cancer Cell. 2023 Mar 13;41(3):374-403. doi: 10.1016/j.ccell.2023.02.016.
5
Novel Roles of Nanog in Cancer Cells and Their Extracellular Vesicles.Nanog 在癌细胞及其细胞外囊泡中的新作用。
Cells. 2022 Dec 1;11(23):3881. doi: 10.3390/cells11233881.
6
The extracellular matrix protein fibulin-3/EFEMP1 promotes pleural mesothelioma growth by activation of PI3K/Akt signaling.细胞外基质蛋白纤连蛋白-3/表皮生长因子样模块包含蛋白1通过激活PI3K/Akt信号传导促进胸膜间皮瘤生长。
Front Oncol. 2022 Oct 11;12:1014749. doi: 10.3389/fonc.2022.1014749. eCollection 2022.
7
ECM Mechanoregulation in Malignant Pleural Mesothelioma.恶性胸膜间皮瘤中的细胞外基质机械调节
Front Bioeng Biotechnol. 2022 Feb 14;10:797900. doi: 10.3389/fbioe.2022.797900. eCollection 2022.
8
A panel of emerging EMT genes identified in malignant mesothelioma.一组在恶性间皮瘤中鉴定出的新兴 EMT 基因。
Sci Rep. 2022 Jan 19;12(1):1007. doi: 10.1038/s41598-022-04973-x.
9
Hallmarks of Cancer: New Dimensions.癌症的特征：新视角。
Cancer Discov. 2022 Jan;12(1):31-46. doi: 10.1158/2159-8290.CD-21-1059.
10
CD44: A Multifunctional Mediator of Cancer Progression.CD44：癌症进展的多功能介质。
Biomolecules. 2021 Dec 9;11(12):1850. doi: 10.3390/biom11121850.