Men Hongsheng
Mutant Mouse Resource and Research Center, Columbia, MO 65201, USA.
Rat Resource and Research Center, Columbia, MO 65201, USA.
Biology (Basel). 2024 Oct 1;13(10):789. doi: 10.3390/biology13100789.
Assisted reproductive technology has revolutionized our ability to genetically manipulate, maintain and rederive laboratory animals of biomedical importance; manipulate animal reproduction or genetics to boost production of farm animals; and improve human reproductive health. The media for in vitro manipulation and the culture of embryos play a critical role in the development of assisted reproductive technology. In this review, the evolution of culture media supporting embryo development in vitro from selected animal species, laboratory animals (mice and rats) and farm animals (pigs and cattle), will be discussed with a focus on the development of chemically defined media.
辅助生殖技术彻底改变了我们对具有生物医学重要性的实验动物进行基因操作、保存和重新培育的能力；通过操纵动物繁殖或基因来提高农场动物的产量；以及改善人类生殖健康。体外操作和胚胎培养的培养基在辅助生殖技术的发展中起着关键作用。在这篇综述中，我们将讨论支持选定动物物种、实验动物（小鼠和大鼠）以及农场动物（猪和牛）体外胚胎发育的培养基的演变，重点是化学成分确定的培养基的发展。
