Brusentsev E Iu, Igonina T N, Amstislavskiĭ S Ia
Ontogenez. 2014 Mar-Apr;45(2):73-88.
This review covers the basic principles and methods of in vitro culture of preimplantation mammalian embryos. The features of in vitro development of embryos of various species of animals with allowance for the composition of nutrient media are described, with special attention paid to those species that have traditionally been consideredas laboratory (i.e., mice, rats, and hamsters). The effects of suboptimal culturing conditions of preimplantation embryos on the formation of the phenotype of individuals developed from these embryos are discussed. New approaches to optimize the conditions of the development of preimplantation mammalian embryos in vitro are analyzed.
本综述涵盖了哺乳动物植入前胚胎体外培养的基本原理和方法。描述了不同动物物种胚胎在体外发育的特点，并考虑了营养培养基的成分，特别关注了那些传统上被视为实验动物的物种（即小鼠、大鼠和仓鼠）。讨论了植入前胚胎培养条件欠佳对由这些胚胎发育而来的个体表型形成的影响。分析了优化哺乳动物植入前胚胎体外发育条件的新方法。
