Bufan Biljana, Marčetić Mirjana, Djuretić Jasmina, Ćuruvija Ivana, Blagojević Veljko, Božić Dragana D, Milutinović Violeta, Janković Radmila, Sopta Jelena, Kotur-Stevuljević Jelena, Arsenović-Ranin Nevena
Department of Microbiology and Immunology, University of Belgrade-Faculty of Pharmacy, 11221 Belgrade, Serbia.
Department of Pharmacognosy, University of Belgrade-Faculty of Pharmacy, 11221 Belgrade, Serbia.
Biology (Basel). 2024 Oct 12;13(10):818. doi: 10.3390/biology13100818.
The anti-inflammatory/immunomodulatory effects of L. (TM), a plant distributed in the Mediterranean region, have been insufficiently examined. The effects of the TM ethanol extract were tested in a rat collagen-induced arthritis (CIA) model of rheumatoid arthritis. LC-MS was used for the phytochemical analysis of the TM extract. rats were immunized with bovine type II collagen (CII) in incomplete Freund's adjuvant for CIA, and treated with 100 or 200 mg/kg of TM extract daily via oral administration. Clinical and histopathological evaluations and a flow cytometric analysis of the phenotypic and functional characteristics of splenocytes and draining lymph node cells were performed. The cytokines in the paw tissue culture supernatants and anti-CII antibodies in serum were determined by ELISA. The TM extract, with the dominant components verbascoside and luteolin 7--rutinoside, reduced the arthritic score and ankle joint inflammation in CIA rats, promoted the antioxidant profile in serum, and lowered pro-inflammatory TNF-α, IL-6 and IL-1β production. It suppressed the activation status of CD11b+ cells by lowering CD86, MHCII and TLR-4 expression, and promoted the Th17/T regulatory cell (Tregs) balance towards Tregs. A lower frequency of B cells was accompanied by a lower level of anti-CII antibodies in treated rats. These findings imply the favorable effect of TM extract on the clinical presentation of CIA, suggesting its anti-inflammatory/immunomodulatory action and potential therapeutic effect.
分布于地中海地区的一种植物L. (TM) 的抗炎/免疫调节作用尚未得到充分研究。在类风湿性关节炎的大鼠胶原诱导性关节炎 (CIA) 模型中测试了TM乙醇提取物的作用。采用液相色谱-质谱联用 (LC-MS) 对TM提取物进行植物化学分析。用牛II型胶原 (CII) 在不完全弗氏佐剂中免疫大鼠以诱导CIA，并通过口服每日给予100或200 mg/kg的TM提取物进行治疗。进行了临床和组织病理学评估以及对脾细胞和引流淋巴结细胞的表型和功能特征的流式细胞术分析。通过酶联免疫吸附测定 (ELISA) 测定爪组织培养上清液中的细胞因子和血清中的抗CII抗体。以毛蕊花糖苷和木犀草素7-芸香糖苷为主要成分的TM提取物降低了CIA大鼠的关节炎评分和踝关节炎症，促进了血清中的抗氧化状态，并降低了促炎细胞因子TNF-α、IL-6和IL-1β的产生。它通过降低CD86、MHCII和TLR-4的表达抑制了CD11b+细胞的活化状态，并促进了Th17/调节性T细胞 (Tregs) 平衡向Tregs倾斜。在接受治疗的大鼠中，较低频率的B细胞伴随着较低水平的抗CII抗体。这些发现表明TM提取物对CIA的临床表现具有有益作用，提示其抗炎/免疫调节作用和潜在的治疗效果。
