纤毛虫捕食对[具体亚种名称]接合作用的影响。（你提供的原文中“subsp.”后面缺少具体内容，这里只能按格式翻译并补充说明一下。）

Impact of Predation by Ciliate on Conjugation in subsp. .

作者信息

Durocher Alicia F, Paquet Valérie E, St-Laurent Rébecca E, Duchaine Caroline, Charette Steve J

机构信息

Institut de Biologie Intégrative et des Systèmes (IBIS), Université Laval, Québec, QC G1V 0A6, Canada.

Centre de Recherche de l'Institut Universitaire de Cardiologie et de Pneumologie de Québec (IUCPQ), Université Laval, Québec, QC G1V 4G5, Canada.

出版信息

Antibiotics (Basel). 2024 Oct 11;13(10):960. doi: 10.3390/antibiotics13100960.

DOI:10.3390/antibiotics13100960

PMID:39452226

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11504919/

Abstract

BACKGROUND/OBJECTIVES: Antibiotic resistance gene (ARG) spread is driven by horizontal gene transfer (HGT). Ciliated protozoa may contribute to this process, as their predation has been shown to facilitate HGT in certain bacteria. Here, this phenomenon was further investigated using subsp. . This fish pathogen bears an extensive and dynamic plasmidome, suggesting a high potential for HGT.

METHODS

strains carrying one of three conjugative plasmids bearing ARGs (pSN254b, pRAS1b or pAsa4b) were cocultured with a recipient, either , or . Conjugation rates were assessed in the presence and absence of the ciliate . PCR genotyping confirmed the acquisition of the conjugative plasmids and was used to verify the mobilization of other plasmids.

RESULTS

The basal rate of conjugation observed was high. Under the conditions studied, ciliate predation did not appear to influence the conjugation rate, except at higher proportions of ciliates, which typically hampered conjugation. Microscopy revealed that most bacteria were digested in these conditions. PCR screening demonstrated that small mobilizable plasmids from (pAsa1, pAsa2, pAsa3, and pAsal1) were acquired by the recipients along with the conjugative plasmids, with a slight effect of the ciliates in some donor/recipient cell combination.

CONCLUSIONS

These results highlight how can conjugate efficiently with different species and how complex its relationship with ciliates is.

摘要

背景/目的：抗生素抗性基因（ARG）的传播是由水平基因转移（HGT）驱动的。纤毛原生动物可能促成这一过程，因为它们的捕食已被证明能促进某些细菌中的水平基因转移。在此，使用亚种进一步研究了这一现象。这种鱼类病原体拥有广泛且动态的质粒组，表明其水平基因转移潜力很高。

方法

携带三种带有抗生素抗性基因的接合性质粒之一（pSN254b、pRAS1b或pAsa4b）的菌株与受体菌（分别为、或）共培养。在有和没有纤毛虫的情况下评估接合率。PCR基因分型证实了接合性质粒的获得，并用于验证其他质粒的转移。

结果

观察到的基础接合率很高。在所研究的条件下，纤毛虫捕食似乎并未影响接合率，除非纤毛虫比例较高，而这通常会阻碍接合。显微镜检查显示，在这些条件下大多数细菌被消化。PCR筛选表明，受体菌在获得接合性质粒的同时，还获得了来自的小型可移动质粒（pAsa1、pAsa2、pAsa3和pAsal1），在某些供体/受体细胞组合中纤毛虫有轻微影响。