Wang Yu, Wen Fengyi, Zhou Xiangyi, Chen Guoxing, Tian Chunxia, Qian Jiali, Wu Huiming, Chen Mengli
Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, China.
Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.
Insects. 2024 Sep 24;15(10):733. doi: 10.3390/insects15100733.
(Diptera: Drosophilidae), spotted-wing drosophila, poses a significant threat to soft-skinned fruit crops in the Americas, Europe, Africa, and Oceania, as well as in Asia. The application of chemical insecticides is the primary control strategy for ; however, resistance has developed with the indiscriminate use of chemical insecticides. Essential oils, considered potential alternatives to pesticidal strategies, exhibit potent toxic and sublethal behavioral effects against numerous pests, including . Clary sage oil repels a variety of agricultural and household pests; however, whether it has a repellent effect against remains unknown. Here, we found that clary sage oil exhibited dose-dependent repellency against adults in a T-maze assay, a two-choice assay and a two-choice attraction assay. Also, clary sage oil showed a significant repellent effect against larvae. Next, we explored the chemical constituents of clary sage oil by GC-MS and identified two major constituents, linalyl acetate (40.03%) and linalool (23.02%). Furthermore, the behavioral assays of linalyl acetate and linalool showed that both compounds conferred comparable repellency against adults and larvae. Finally, we found clary sage oil, linalyl acetate, and linalool elicited EAG responses in , especially clary sage oil, suggesting the repellency was mediated by the olfactory system. These findings indicate that shows olfactory-based behavioral avoidance of clary sage oil, linalyl acetate, and linalool. Clary sage oil and its major constituents may be possible alternatives in the management of .
（双翅目：果蝇科）斑翅果蝇对美洲、欧洲、非洲、大洋洲以及亚洲的软皮水果作物构成了重大威胁。化学杀虫剂的应用是其主要防治策略；然而，由于化学杀虫剂的滥用，果蝇已产生抗性。香精油被认为是杀虫策略的潜在替代品，对包括斑翅果蝇在内的众多害虫具有强大的毒性和亚致死行为影响。鼠尾草精油能驱避多种农业和家庭害虫；然而，其对斑翅果蝇是否具有驱避作用仍不清楚。在此，我们发现鼠尾草精油在T型迷宫试验、二选一试验和二选一吸引试验中对斑翅果蝇成虫表现出剂量依赖性驱避作用。此外，鼠尾草精油对斑翅果蝇幼虫也显示出显著的驱避效果。接下来，我们通过气相色谱 - 质谱联用仪（GC - MS）探究了鼠尾草精油的化学成分，并鉴定出两种主要成分，乙酸芳樟酯（40.03%）和芳樟醇（23.02%）。此外，乙酸芳樟酯和芳樟醇的行为试验表明，这两种化合物对斑翅果蝇成虫和幼虫具有相当的驱避作用。最后，我们发现鼠尾草精油、乙酸芳樟酯和芳樟醇能引起斑翅果蝇的触角电位（EAG）反应，尤其是鼠尾草精油，这表明驱避作用是由嗅觉系统介导的。这些发现表明，斑翅果蝇对鼠尾草精油、乙酸芳樟酯和芳樟醇表现出基于嗅觉的行为回避。鼠尾草精油及其主要成分可能是斑翅果蝇防治中的潜在替代品。
