Center for Agroforestry Mega Data Science, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Virus Genes. 2023 Apr;59(2):338-342. doi: 10.1007/s11262-022-01962-0. Epub 2022 Dec 12.
The aster leafhopper Macrosteles fascifrons is a common insect pest that feeds on rice and other plants and may serve as a vector to transmit various viruses. Here, we discovered a novel virus from M. fascifrons using metagenomic sequencing. We obtained its complete genome sequence by contig assembly and rapid amplification of cDNA ends, and verified the genome sequence by Sanger sequencing of overlapping segments. Based on homology search and phylogenetic analysis, the new virus belongs to the family Iflaviridae and it is tentatively named "Macrosteles fascifrons iflavirus 1" (MfIV1). Excluding the poly(A) tail, the MfIV1 genome is 10,581 nucleotides in length and it is predicted to encode a polyprotein of 3119 amino acids long, which is likely further processed to several polypeptides with conserved domains, including two rhinovirus like (rhv-like) capsid domains, a cricket paralysis virus (CRPV) capsid domain, a helicase domain, and an RNA-dependent RNA polymerase (RdRp) domain. BLAST searches show that the highest amino acid sequence identity between the capsid proteins of MfIV1 and those of other reported iflaviruses is 60.22%, indicating that MfIV1 is a new member in the family Iflaviridae.
星叶蝉 Macrosteles fascifrons 是一种常见的昆虫害虫，以水稻和其他植物为食，可能作为传播各种病毒的媒介。在这里，我们使用宏基因组测序从 M. fascifrons 中发现了一种新型病毒。我们通过连续序列组装和快速 cDNA 末端扩增获得了其完整的基因组序列，并通过重叠片段的 Sanger 测序验证了基因组序列。基于同源搜索和系统发育分析，该新病毒属于 Iflaviridae 科，暂命名为“Macrosteles fascifrons iflavirus 1”（MfIV1）。不包括 poly(A) 尾，MfIV1 基因组长 10581 个核苷酸，预测编码一个 3119 个氨基酸长的多聚蛋白，该多聚蛋白可能进一步加工成几个具有保守结构域的多肽，包括两个类似鼻病毒（rhv-like）衣壳结构域、蟋蟀麻痹病毒（CRPV）衣壳结构域、解旋酶结构域和 RNA 依赖性 RNA 聚合酶（RdRp）结构域。BLAST 搜索表明，MfIV1 的衣壳蛋白与其他已报道的 Iflaviridae 病毒的衣壳蛋白之间的最高氨基酸序列同一性为 60.22%，表明 MfIV1 是 Iflaviridae 科的一个新成员。
