Esmaeili A, Chandra T, Schlatterer K
Department of Pediatric Cardiology, Johann Wolfgang Goethe University Hospital, 60590 Frankfurt am Main,; Germany.
Anticancer Res. 2007 Jul-Aug;27(4B):2197-207.
A host-mediated assay system for detection of the transforming activity of different chemical carcinogens on peritoneal macrophages has been previously established. Directly, as well as indirectly acting carcinogenic substances administered intraperitoneally to NMRI mice could be examined in this way. Resident macrophages were recovered by peritoneal lavage from treated and untreated mice and cultured in soft agar. After 5-6 days normal and transformed cells could be distinguished. By the use of this system an immortalized macrophage-like cell line was derived from the peritoneal cells of NMRI mice treated with 17alpha-ethinylestradiol. This cell line enabled us to perform additional investigations on the underlying molecular effects of 17alpha-ethinylestradiol, such as detection of the transformation specific polypeptides as surrogate markers for transformation. The investigation reported here describes the cell-transforming and oncogenic potential of 17alpha-ethinylestradiol.
先前已建立了一种宿主介导的检测系统，用于检测不同化学致癌物对腹膜巨噬细胞的转化活性。通过这种方式，可以检测直接或间接作用的致癌物质经腹腔注射给NMRI小鼠后的情况。通过腹腔灌洗从处理过和未处理过的小鼠中回收驻留巨噬细胞，并在软琼脂中培养。5-6天后，可以区分正常细胞和转化细胞。通过使用该系统，从用17α-乙炔雌二醇处理的NMRI小鼠的腹膜细胞中获得了一种永生化的巨噬细胞样细胞系。该细胞系使我们能够对17α-乙炔雌二醇的潜在分子效应进行进一步研究，例如检测转化特异性多肽作为转化的替代标志物。此处报道的研究描述了17α-乙炔雌二醇的细胞转化和致癌潜力。
