García-Sánchez Víctor Javier, Sánchez-López Karina Lizbeth, Esquivel Méndez Juana Jazmín, Sánchez-Hernández Daniel, Cervantes-Chávez José Antonio, Landeros-Jaime Fidel, Mendoza-Mendoza Artemio, Vega-Arreguín Julio Cesar, Esquivel-Naranjo Edgardo Ulises
Unit for Basic and Applied Microbiology, Faculty of Natural Sciences, Autonomous University of Queretaro, Queretaro 76140, Mexico.
Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New Zealand.
J Fungi (Basel). 2024 Sep 26;10(10):671. doi: 10.3390/jof10100671.
Parasitic species of use hydrolytic enzymes to destroy the host cell wall. Preferent carbon and nitrogen sources suppress the expression of genes related to parasitism. Here, different nutrients were evaluated in the parasitic isolated NI-1, which was identified as . The genes and (cellobiohydrolases), (endoglucanase), and and (proteinases) were poorly expressed during the interaction between NI-1 and on PDA. However, gene expression improved on minimal medium with preferent and alternative carbon sources. Dextrin and glucose stimulated higher transcript levels than cellulose, sucrose, and glycerol. Also, ammonium stimulated a stronger parasitic responsiveness than the alternative nitrogen sources. During interaction against different phytopathogens, NI-1 detects their host differentially from a distance due to the and genes being only induced by . The and genes were induced before contact with and , while when confronted with they were stimulated until contact and overgrowth. The and genes were induced before contact against the three-host assayed. Overall, prefers to parasitize and has the capacity to distinguish between an oomycete and a fungus, but nutrient quality regulates its parasitic responsiveness.
寄生菌种利用水解酶破坏宿主细胞壁。优先的碳源和氮源会抑制与寄生相关基因的表达。在此，对寄生分离株NI-1（已鉴定为[具体菌种名称未给出]）中的不同营养物质进行了评估。在NI-1与[具体菌种名称未给出]在马铃薯葡萄糖琼脂（PDA）上相互作用期间，基因[具体基因名称未给出]和[具体基因名称未给出]（纤维二糖水解酶）、[具体基因名称未给出]（内切葡聚糖酶）以及[具体基因名称未给出]和[具体基因名称未给出]（蛋白酶）表达水平较低。然而，在含有优先和替代碳源的基本培养基上，基因表达有所改善。糊精和葡萄糖刺激的转录水平高于纤维素、蔗糖和甘油。此外，铵盐刺激的寄生反应性比替代氮源更强。在与不同植物病原体相互作用期间，由于[具体基因名称未给出]和[具体基因名称未给出]基因仅由[具体诱导物未给出]诱导，NI-1能在一定距离外对其宿主进行差异检测。[具体基因名称未给出]和[具体基因名称未给出]基因在与[具体病原体名称未给出]和[具体病原体名称未给出]接触前被诱导，而当面对[具体病原体名称未给出]时，它们在接触和过度生长之前一直受到刺激。[具体基因名称未给出]和[具体基因名称未给出]基因在与所检测的三种宿主接触前被诱导。总体而言，[具体菌种名称未给出]倾向于寄生，并且有能力区分卵菌和真菌，但营养质量会调节其寄生反应性。
