Research Area Gene Technology, Institute of Chemical Engineering, Vienna University of Technology, Gumpendorferstraße 1a/166, A-1060 Vienna, Austria.
Appl Environ Microbiol. 2011 Jul;77(13):4361-70. doi: 10.1128/AEM.00129-11. Epub 2011 Apr 29.
A high-throughput sequencing approach was utilized to carry out a comparative transcriptome analysis of Trichoderma atroviride IMI206040 during mycoparasitic interactions with the plant-pathogenic fungus Rhizoctonia solani. In this study, transcript fragments of 7,797 Trichoderma genes were sequenced, 175 of which were host responsive. According to the functional annotation of these genes by KOG (eukaryotic orthologous groups), the most abundant group during direct contact was "metabolism." Quantitative reverse transcription (RT)-PCR confirmed the differential transcription of 13 genes (including swo1, encoding an expansin-like protein; axe1, coding for an acetyl xylan esterase; and homologs of genes encoding the aspartyl protease papA and a trypsin-like protease, pra1) in the presence of R. solani. An additional relative gene expression analysis of these genes, conducted at different stages of mycoparasitism against Botrytis cinerea and Phytophthora capsici, revealed a synergistic transcription of various genes involved in cell wall degradation. The similarities in expression patterns and the occurrence of regulatory binding sites in the corresponding promoter regions suggest a possible analog regulation of these genes during the mycoparasitism of T. atroviride. Furthermore, a chitin- and distance-dependent induction of pra1 was demonstrated.
采用高通量测序方法对木霉属真菌 IMI206040 在与其植物病原菌立枯丝核菌互作过程中的转录组进行了比较分析。在这项研究中，测序了 7797 个木霉属基因的转录片段，其中 175 个对宿主有响应。根据这些基因的 COG（真核生物直系同源群）功能注释，在直接接触时最丰富的组是“代谢”。定量反转录（RT）-PCR 证实了在存在立枯丝核菌的情况下，13 个基因（包括编码扩张蛋白样蛋白的 swo1、编码乙酰木聚糖酯酶的 axe1 以及编码天冬氨酸蛋白酶 papA 和胰蛋白酶样蛋白酶 pra1 的基因的同源物）的转录差异。对这些基因在木霉属真菌对灰葡萄孢和辣椒疫霉的生防作用的不同阶段进行的额外相对基因表达分析表明，参与细胞壁降解的各种基因的协同转录。表达模式的相似性和相应启动子区域中调控结合位点的出现表明，在木霉属真菌的生防过程中，这些基因可能存在类似的调控。此外，还证明了 pra1 依赖于几丁质和距离的诱导。
