Stakheev Alexander A, Taliansky Michael, Kalinina Natalia O, Zavriev Sergey K
M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, 117997 Moscow, Russia.
A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, Russia.
J Fungi (Basel). 2024 Sep 29;10(10):682. doi: 10.3390/jof10100682.
Mycotoxin contamination of food and feed is a worldwide problem that needs to be addressed with highly efficient and biologically safe techniques. RNA interference (RNAi) is a natural mechanism playing an important role in different processes in eukaryotes, including the regulation of gene expression, maintenance of genome stability, protection against viruses and others. Recently, RNAi-based techniques have been widely applied for the purposes of food safety and management of plant diseases, including those caused by mycotoxin-producing fungi. In this review, we summarize the current state-of-the-art RNAi-based approaches for reducing the aggressiveness of key toxigenic fungal pathogens and mycotoxin contamination of grain and its products. The ways of improving RNAi efficiency for plant protection and future perspectives of this technique, including progress in methods of double-stranded RNA production and its delivery to the target cells, are also discussed.
食品和饲料中的霉菌毒素污染是一个全球性问题，需要采用高效且生物安全的技术加以解决。RNA干扰（RNAi）是一种自然机制，在真核生物的不同过程中发挥着重要作用，包括基因表达调控、基因组稳定性维持、抗病毒防御等。近年来，基于RNAi的技术已广泛应用于食品安全和植物病害管理，包括由产霉菌毒素真菌引起的病害。在本综述中，我们总结了当前基于RNAi的最先进方法，这些方法可降低关键产毒真菌病原体的侵袭性以及谷物及其产品中的霉菌毒素污染。我们还讨论了提高RNAi对植物保护效率的方法以及该技术的未来前景，包括双链RNA生产方法及其向靶细胞递送方面的进展。
