Institute of Phytopathology, Research Centre for BioSystems, Land Use and Nutrition, Justus Liebig University Giessen, Giessen, Germany.
Institut de Biologie Moléculaire des Plantes, CNRS, Université de Strasbourg, Strasbourg, France.
Mol Plant Pathol. 2024 Oct;25(10):e70011. doi: 10.1111/mpp.70011.
Fusarium fungi are a pervasive threat to global agricultural productivity. They cause a spectrum of plant diseases that result in significant yield losses and threaten food safety by producing mycotoxins that are harmful to human and animal health. In recent years, the exploitation of the RNA interference (RNAi) mechanism has emerged as a promising avenue for the control of Fusarium-induced diseases, providing both a mechanistic understanding of Fusarium gene function and a potential strategy for environmentally sustainable disease management. However, despite significant progress in elucidating the presence and function of the RNAi pathway in different Fusarium species, a comprehensive understanding of its individual protein components and underlying silencing mechanisms remains elusive. Accordingly, while a considerable number of RNAi-based approaches to Fusarium control have been developed and many reports of RNAi applications in Fusarium control under laboratory conditions have been published, the applicability of this knowledge in agronomic settings remains an open question, and few convincing data on RNAi-based disease control under field conditions have been published. This review aims to consolidate the current knowledge on the role of RNAi in Fusarium disease control by evaluating current research and highlighting important avenues for future investigation.
镰刀菌真菌是全球农业生产力的普遍威胁。它们会引起一系列植物疾病，导致产量显著下降，并通过产生对人类和动物健康有害的真菌毒素来威胁食品安全。近年来，RNA 干扰 (RNAi) 机制的开发已成为控制镰刀菌诱导疾病的一种有前途的方法，为了解镰刀菌基因功能提供了机制，并为环境可持续的疾病管理提供了潜在策略。然而，尽管在不同的镰刀菌物种中阐明 RNAi 途径的存在和功能方面取得了重大进展，但对其单个蛋白质成分和潜在沉默机制的全面了解仍难以捉摸。因此，尽管已经开发了许多基于 RNAi 的镰刀菌控制方法，并且已经发表了许多关于 RNAi 在镰刀菌控制中的实验室条件下应用的报告，但该知识在农业环境中的适用性仍然是一个悬而未决的问题，并且很少有令人信服的田间条件下基于 RNAi 的疾病控制数据发表。本综述旨在通过评估当前的研究工作，强调未来研究的重要方向，从而整合当前关于 RNAi 在镰刀菌疾病控制中的作用的知识。
