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染色体级别的基因组和转录组分析揭示了……中萜类次生代谢产物的差异调控 。 （注：原文句末不完整，缺少具体所指对象）

Chromosome-Scale Genome and Transcriptomic Analyses Reveal Differential Regulation of Terpenoid Secondary Metabolites in .

作者信息

Meng Kexin, Lv Junyi, Zhang Tuo, Liu Yuanyuan, Zhang Peng, Zhang Yue, Hu Banghui, Huang Qianhui, Xie Baogui, Fu Junsheng

机构信息

College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

Mycological Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

出版信息

J Fungi (Basel). 2024 Oct 9;10(10):704. doi: 10.3390/jof10100704.

DOI:10.3390/jof10100704
PMID:39452656
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11508549/
Abstract

Construction of the genome of , a species of edible mushroom, and identification of the genes involved in terpenoid biosynthesis can determine the biology and genetics of terpenoids. The present study describes the assembly of a high-quality chromosome-scale genome of using Pacbio HiFi sequencing and Hi-C technology. This genome consisted of 13 chromosomes, a total size of 43.6 Mb, contigs of N50 3.6 Mb, GC content at 54%, and BUSCOs integrity of 96.9%. Genes associated with terpenoid biosynthesis were predicted by KEGG enrichment analysis and homologous alignment. The and genes, encoding proteins in the terpenoid backbone synthesis pathway, were found to encode geranylgeranyl pyrophosphate and farnesyl diphosphate synthases, key enzymes in the biosynthesis of geranylgeranyl diphosphate, a precursor of several diterpenoids. was found to be involved in regulating diterpene cyclase. The , , , and genes were found to encode sesquiterpene synthesis. Most of these genes were more highly expressed in dikaryotic mycelia than in the primordium and fruiting bodies, indicating that terpenoids may be more abundant in dikaryotic mycelia. To our knowledge, this study is the first to assemble the genome at the chromosome scale and to identify the genes involved in terpenoid biosynthesis.

摘要

食用蘑菇物种的基因组构建以及萜类生物合成相关基因的鉴定能够确定萜类化合物的生物学特性和遗传学特征。本研究描述了利用Pacbio HiFi测序和Hi-C技术组装高质量染色体水平的[蘑菇名称]基因组。该基因组由13条染色体组成，总大小为43.6 Mb，N50重叠群为3.6 Mb，GC含量为54%，BUSCO完整性为96.9%。通过KEGG富集分析和同源比对预测了与萜类生物合成相关的基因。在萜类骨架合成途径中编码蛋白质的[基因名称1]和[基因名称2]基因，被发现分别编码香叶基香叶基焦磷酸合酶和法呢基二磷酸合酶，这两种酶是几种二萜类化合物前体香叶基香叶基焦磷酸生物合成中的关键酶。[基因名称3]被发现参与调控二萜环化酶。[基因名称4]、[基因名称5]、[基因名称6]和[基因名称7]基因被发现编码倍半萜合成。这些基因中的大多数在双核菌丝体中的表达水平高于原基和子实体，表明萜类化合物在双核菌丝体中可能更为丰富。据我们所知，本研究首次在染色体水平上组装了[蘑菇名称]基因组，并鉴定了参与萜类生物合成的基因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c71/11508549/d885ad28da5d/jof-10-00704-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c71/11508549/b8bb9ed4cdbc/jof-10-00704-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c71/11508549/be8ba5147e43/jof-10-00704-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c71/11508549/ec650dd02b04/jof-10-00704-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c71/11508549/d37034f50e00/jof-10-00704-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c71/11508549/a1ed049a43f1/jof-10-00704-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c71/11508549/c21b8d271fa3/jof-10-00704-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c71/11508549/2e9b9b723dc6/jof-10-00704-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c71/11508549/d885ad28da5d/jof-10-00704-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c71/11508549/b8bb9ed4cdbc/jof-10-00704-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c71/11508549/be8ba5147e43/jof-10-00704-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c71/11508549/ec650dd02b04/jof-10-00704-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c71/11508549/d37034f50e00/jof-10-00704-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c71/11508549/a1ed049a43f1/jof-10-00704-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c71/11508549/c21b8d271fa3/jof-10-00704-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c71/11508549/2e9b9b723dc6/jof-10-00704-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c71/11508549/d885ad28da5d/jof-10-00704-g009.jpg

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