Zhang Caixia, Xu Lijun, Li Jian, Chen Jiansong, Yang Manjun
Tibet Vocational Technical College, Lhasa, Xizang, 850000, China.
Tibet University of Tibetan Medicine, Lhasa, Xizang, 850000, China.
Int J Genomics. 2022 Jan 31;2022:4017654. doi: 10.1155/2022/4017654. eCollection 2022.
The fruiting bodies or mycelia of () contain many physiologically active compounds that are used to treat various diseases, including cardiovascular disorders and cancers. However, the genome of has not been sequenced, which hinders further investigations into aspects, such as bioactivity or evolutionary events. The present study is aimed at (i) performing sequencing of the assembled genome; (ii) mapping the reads from PE400 DNA into the assembled genome; (iii) identifying the full length of all the repeated sequences; and (iv) annotating protein-coding genes using GO, eggNOG, and KEGG databases. The assembled genome comprised 5,59,05,675 bp, including 307 contigs. The mapping rate of reads obtained from PE400 DNA in the assembled genome was 92.46%. We identified 2,525 repeated sequences of 14,23,274 bp length. We predicted ncRNAs of 48,895 bp and 11,736 genes encoding proteins that were annotated in the GO, eggNOG, and KEGG databases. We are the first to sequence the entire genome (NCBI; Assembly: ASM367540v1), which will serve as a reference for studying the evolutionary diversification of edible and medicinal mushrooms and facilitate the application of bioactivity in .
（）的子实体或菌丝体含有许多生理活性化合物，可用于治疗各种疾病，包括心血管疾病和癌症。然而，（）的基因组尚未测序，这阻碍了对其生物活性或进化事件等方面的进一步研究。本研究旨在：（i）对组装好的基因组进行测序；（ii）将来自PE400 DNA的 reads 映射到组装好的基因组中；（iii）鉴定所有重复序列的全长；（iv）使用GO、eggNOG和KEGG数据库注释蛋白质编码基因。组装好的基因组由55905675 bp组成，包括307个重叠群。从PE400 DNA获得的reads在组装好的基因组中的映射率为92.46%。我们鉴定出2525个长度为1423274 bp的重复序列。我们预测了长度为48895 bp的非编码RNA和11736个编码蛋白质的基因，并在GO、eggNOG和KEGG数据库中进行了注释。我们是第一个对整个（）基因组进行测序的（NCBI；组装：ASM367540v1），这将为研究食用和药用蘑菇的进化多样性提供参考，并促进（）中生物活性的应用。
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