Szczuka Ewa, Rolnicka Dominika, Wesołowska Maria
Department of Microbiology, Faculty of Biology, Institute of Experimental Biology, Adam Mickiewicz University in Poznań, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland.
Microbiology Laboratory, University Clinical Hospital in Poznań, ul. Przybyszewskiego 49, 60-355 Poznań, Poland.
Pathogens. 2024 Sep 25;13(10):827. doi: 10.3390/pathogens13100827.
Vancomycin-resistant enterococci (VRE) are considered one of the main nosocomial pathogens due to their increasing antibiotic resistance and ability to cause life-threatening infections in humans. This study included VRE isolates obtained from various specimens including urine, blood, faeces, wounds, sputum, and oral cavity wash. Of the 37 strains, 30 (81.1%) and 7 (18.9%) were identified by MALDI TOF as and , respectively. The clinical vancomycin-resistant enterococci exhibited multi-drug resistance (MDR). Apart from vancomycin, the enterococci exhibited resistance to penicillins (89.1 to 100%), fluoroquinolones (100%), rifampicin (86.5%), tetracycline (27%), aminoglycosides (56.8 to 86.5%), quinupristin-dalfopristin (35.1%), and chloramphenicol (10.8%). Moreover, resistance to linezolid and tigecycline emerged among the tested vancomycin-resistant enterococci. The analysis of aminoglycoside modifying enzyme (AME) genes showed the presence of bifunctional '″ genes contributed to high-level aminoglycoside resistance (HLAR) in the and isolates. The other AME gene, i.e., ', was also found in the VRE isolates. All strains carried the gene. Enterococci from colonised gastrointestinal tracts (1/2.7%) and from infection (6/16.2%) showed cytotoxic activity against the human epithelial cell line HEp-2.
耐万古霉素肠球菌（VRE）因其不断增加的抗生素耐药性以及在人类中引发危及生命感染的能力，被视为主要的医院病原体之一。本研究纳入了从尿液、血液、粪便、伤口、痰液和口腔冲洗液等各种标本中分离出的VRE菌株。在37株菌株中，基质辅助激光解吸电离飞行时间质谱（MALDI TOF）分别鉴定出30株（81.1%）和7株（18.9%）为[具体菌种1]和[具体菌种2]。临床耐万古霉素肠球菌表现出多重耐药（MDR）。除万古霉素外，肠球菌对青霉素（89.1%至100%）、氟喹诺酮类（100%）、利福平（86.5%）、四环素（27%）、氨基糖苷类（56.8%至86.5%）、奎奴普丁-达福普汀（35.1%）和氯霉素（10.8%）均有耐药性。此外，在测试的耐万古霉素肠球菌中出现了对利奈唑胺和替加环素的耐药性。氨基糖苷类修饰酶（AME）基因分析显示，在[具体菌种1]和[具体菌种2]分离株中存在双功能的[具体基因名称]基因，这导致了高水平氨基糖苷类耐药（HLAR）。在VRE分离株中还发现了另一个AME基因即[具体基因名称]。所有菌株均携带[具体基因名称]基因。来自定植胃肠道的肠球菌（1/2.7%）和感染的肠球菌（6/16.2%）对人上皮细胞系HEp-2表现出细胞毒性活性。
