Łukasiewicz Research Network-Łódź Institute of Technology, Marii Sklodowskiej-Curie 19/27, 90-570 Lodz, Poland.
Institute of Polymer and Dye Technology, Faculty of Chemistry, Lodz University of Technology, Stefanowskiego 16, 90-537 Lodz, Poland.
Mar Drugs. 2024 Sep 26;22(10):436. doi: 10.3390/md22100436.
Alginate-based materials have gained significant recognition in the medical industry due to their favorable biochemical properties. As a continuation of our previous studies, we have introduced a new composite consisting of cellulose nonwoven fabric charged with a metallic copper core (CNW-Cu) covered with a calcium alginate (ALGCa) layer. The preparation process for these materials involved three main steps: coating the cellulose nonwoven fabric with copper via magnetron sputtering (CNW → CNW-Cu), subsequent deposition with sodium alginate (CNW-Cu → CNW-Cu/ALGNa), followed by cross-linking the alginate chains with calcium ions (CNW-Cu/ALGNa → CNW-Cu/ALGCa). The primary objective of the work was to supply these composites with such biological attributes as antibacterial and hemostatic activity. Namely, equipping the antibacterial materials (copper action on representative Gram-positive and Gram-negative bacteria and fungal strains) with induction of blood plasma clotting processes (activated partial thromboplastin time (aPTT) and prothrombin time (PT)). We determined the effect of CNW-Cu/ALGCa materials on the viability of Peripheral blood mononuclear (PBM) cells. Moreover, we studied the interactions of CNW-Cu/ALGCa materials with DNA using the relaxation plasmid assay. However, results showed CNW-Cu/ALGCa's cytotoxic properties against PBM cells in a time-dependent manner. Furthermore, the CNW-Cu/ALGCa composite exhibited the potential to interact directly with DNA. The results demonstrated that the CNW-Cu/ALGCa composites synthesized show promising potential for wound dressing applications.
基于海藻酸盐的材料由于其良好的生化特性，在医学领域得到了广泛的认可。作为我们之前研究的延续，我们引入了一种新的复合材料，由带金属铜核的纤维素无纺织物（CNW-Cu）组成，表面覆盖一层海藻酸钠（ALGCa）。这些材料的制备过程包括三个主要步骤：通过磁控溅射在纤维素无纺织物上镀铜（CNW→CNW-Cu），随后用海藻酸钠（CNW-Cu→CNW-Cu/ALGNa）沉积，然后用钙离子交联藻酸盐链（CNW-Cu/ALGNa→CNW-Cu/ALGCa）。这项工作的主要目的是为这些复合材料提供抗菌和止血等生物特性。也就是说，为抗菌材料（铜对代表性的革兰氏阳性和革兰氏阴性细菌和真菌菌株的作用）配备诱导血浆凝固过程的能力（激活部分凝血活酶时间（aPTT）和凝血酶原时间（PT））。我们确定了 CNW-Cu/ALGCa 材料对外周血单个核细胞（PBM）活力的影响。此外，我们使用松弛质粒测定研究了 CNW-Cu/ALGCa 材料与 DNA 的相互作用。然而，结果表明 CNW-Cu/ALGCa 材料对 PBM 细胞具有时间依赖性的细胞毒性。此外，CNW-Cu/ALGCa 复合材料表现出与 DNA 直接相互作用的潜力。结果表明，合成的 CNW-Cu/ALGCa 复合材料具有应用于伤口敷料的巨大潜力。
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