Department of Medical Laboratory Science, College of Health Science, Dankook University, Cheonan-si 31116, Chungcheongnam-do, Republic of Korea.
Marine Bio-Food and Drug Convergence Technology Center, Dankook University, Cheonan-si 31116, Chungcheongnam-do, Republic of Korea.
Mar Drugs. 2024 Oct 19;22(10):479. doi: 10.3390/md22100479.
Atopic dermatitis (AD) is a chronic inflammatory skin disorder associated with significant morbidity, including pruritus, recurrent skin lesions, and immune dysregulation. This study aimed to investigate the antioxidative and anti-AD effects of peptides derived from hydrolyzed (Korea rockfish) tail by-products. Hydrolysates were prepared using various enzymes, including Alcalase, Flavourzyme, Neutrase, and Protamex. Among them, Protamex hydrolysates demonstrated the highest ABTS radical scavenging activity with an RC value of 69.69 ± 0.41 µg/mL. Peptides were further isolated from the Protamex hydrolysate using dialysis, fast protein liquid chromatography (FPLC), and high-performance liquid chromatography (HPLC). The most active peptide, STPO-B-II, exhibited a single peak and was identified as a sequence of Glu-Leu-Ala-Lys-Thr-Trp-His-Asp-Met-Lys, designated as MP003. In vivo experiments were conducted using a 2,4-dinitrochlorbenzene (DNCB)-induced AD model in NC/Nga mice. The isolated peptide, MP003, showed significantly reduced AD symptoms, including erythema, lichenification, and collagen deposition. Additionally, MP003 decreased epidermal and dermal thickness, eosinophil, and mast cell infiltration and downregulated the expression of pro-inflammatory cytokines IL-1β, IL-6, and IgE in serum and skin tissues. These findings suggest that peptides derived from tail by-products may serve as potential therapeutic agents for AD.
特应性皮炎（AD）是一种慢性炎症性皮肤疾病，与显著的发病率相关，包括瘙痒、皮肤病变反复发作和免疫失调。本研究旨在探讨来源于（韩国红娘鱼）鱼尾巴副产物的水解产物的抗氧化和抗 AD 作用。采用不同的酶，包括碱性蛋白酶（Alcalase）、风味蛋白酶（Flavourzyme）、中性蛋白酶（Neutrase）和蛋白酶 X（Protamex），制备了水解产物。其中，Protamex 水解产物的 ABTS 自由基清除活性最高，RC 值为 69.69±0.41µg/mL。进一步采用透析、快速蛋白液相色谱（FPLC）和高效液相色谱（HPLC）从 Protamex 水解产物中分离出肽。最具活性的肽，STPO-B-II，呈现单一峰，并被鉴定为 Glu-Leu-Ala-Lys-Thr-Trp-His-Asp-Met-Lys 序列，命名为 MP003。在 NC/Nga 小鼠 2,4-二硝基氯苯（DNCB）诱导的 AD 模型中进行了体内实验。分离的肽 MP003 显著减轻了 AD 症状，包括红斑、苔藓化和胶原沉积。此外，MP003 降低了表皮和真皮厚度、嗜酸性粒细胞和肥大细胞浸润，并下调了血清和皮肤组织中促炎细胞因子 IL-1β、IL-6 和 IgE 的表达。这些发现表明，来源于红娘鱼尾巴副产物的肽可能是 AD 的潜在治疗剂。
