Department of Medical Laboratory Science, College of Health Science, Dankook University, Cheonan-si 31116, Chungcheongnam-do, Republic of Korea.
Mar Drugs. 2024 Jan 12;22(1):44. doi: 10.3390/md22010044.
This study investigated the antioxidant, antimicrobial, and anti-atopic dermatitis (AD) effects of a novel peptide (CP) derived from a by-product hydrolysate. Alcalase, Flavourzyme, Neutrase, and Protamex enzymes were used to hydrolyze the by-product protein, and the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical-scavenging activity was measured. Alcalase hydrolysate exhibited the highest ABTS radical-scavenging activity, leading to the selection of Alcalase for further purification. The CHAO-1-I fraction, with the highest ABTS activity, was isolated and further purified, resulting in the identification of the peptide CP with the amino acid sequence Ala-Gln-Val-Met-Lys-Leu-Pro-His-Arg-Met-Gln-His-Ser-Gln-Ser. CP demonstrated antimicrobial activity against , inhibiting its growth. In a 2,4-dinitrochlorobenzene (DNCB)-induced AD-like skin model in mice, CP significantly alleviated skin lesions, reduced epidermal and dermal thickness, and inhibited mast cell infiltration. Moreover, CP suppressed the elevated levels of interleukin-6 (IL-6) in the plasma of DNCB-induced mice. These findings highlight the potential of CP as a therapeutic agent for AD and suggest a novel application of this by-product in the fish processing industry.
本研究调查了一种新型肽（CP）的抗氧化、抗菌和抗特应性皮炎（AD）作用，该肽源自一种副产物水解产物。使用 Alcalase、Flavourzyme、Neutrase 和 Protamex 酶水解副产物蛋白，并测量 2,2'-偶氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基清除活性。Alcalase 水解产物表现出最高的 ABTS 自由基清除活性，导致选择 Alcalase 进行进一步纯化。CHAO-1-I 级分具有最高的 ABTS 活性，被分离并进一步纯化，导致鉴定出具有氨基酸序列 Ala-Gln-Val-Met-Lys-Leu-Pro-His-Arg-Met-Gln-His-Ser-Gln-Ser 的肽 CP。CP 对 表现出抗菌活性，抑制其生长。在小鼠 2,4-二硝基氯苯（DNCB）诱导的 AD 样皮肤模型中，CP 显著缓解皮肤损伤，减少表皮和真皮厚度，并抑制肥大细胞浸润。此外，CP 抑制了 DNCB 诱导的小鼠血浆中白细胞介素 6（IL-6）水平的升高。这些发现强调了 CP 作为 AD 治疗剂的潜力，并为鱼类加工业中这种副产物的新应用提供了依据。
