Donnadio Anna, Bini Marzia, Centracchio Catia, Mattarelli Maurizio, Caponi Silvia, Ambrogi Valeria, Pietrella Donatella, Di Michele Alessandro, Vivani Riccardo, Nocchetti Morena
Dipartimento di Scienze Farmaceutiche, Università di Perugia, Via del Liceo, 1, 06123 Perugia, Italy.
Dipartimento di Fisica e Geologia, Università di Perugia, Via A. Pascoli, I-06123 Perugia, Italy.
ACS Biomater Sci Eng. 2021 Apr 12;7(4):1361-1373. doi: 10.1021/acsbiomaterials.0c01643. Epub 2021 Mar 19.
This work is focused on the preparation and multi-technique characterization of potentially biocompatible reactive interfaces obtained by combining layered double hydroxides (LDHs) and hydroxyapatite (HA). Antimicrobial and osteoinductive metallic ions as Zn and Ga were chosen as intralayer constituents of LDH to obtain ZnAl and ZnAlGa systems. These LDHs, exchanged with dihydrogenphosphate anions, promoted the precipitation of HA on the LDH surface yielding HA@LDH composites. X-ray diffraction quantitative analysis, through the Rietveld refinement method, coupled with elemental analysis and micro-Raman spectroscopy showed the formation of a mixed Ca-Zn HA phase. Scanning electron microscopy revealed that HA, in the presence of LDH, grew preferentially along its -axis, thus crystallizing mainly in the form of flake crystals. LDH and HA@LDH composites showed antibacterial activity against and at not cytotoxic concentrations for human osteoblasts (hFob 1.19), especially when Ga cations were present in the LDH structure. The effect of the presence of HA in the composites on the bone-bonding ability and on human osteoblast proliferation was also investigated. The HA seemed to reduce the toxicity of the LDH toward human osteoblast while did not affect the bone-bonding ability. This multidisciplinary study provides the bio-chemical, structural characterization of new LDH and HA@LDH composites, evaluating also their bioactivity to be potentially applicable to titanium-based prostheses.
这项工作聚焦于通过将层状双氢氧化物（LDHs）与羟基磷灰石（HA）相结合而获得的具有潜在生物相容性的反应性界面的制备及多技术表征。选择抗菌和骨诱导金属离子如锌和镓作为LDH的层内成分，以获得ZnAl和ZnAlGa体系。这些与磷酸二氢根阴离子交换的LDHs促进了HA在LDH表面的沉淀，从而产生HA@LDH复合材料。通过Rietveld精修方法进行的X射线衍射定量分析，结合元素分析和显微拉曼光谱，表明形成了混合的Ca-Zn HA相。扫描电子显微镜显示，在LDH存在的情况下，HA优先沿其c轴生长，因此主要以片状晶体的形式结晶。LDH和HA@LDH复合材料对金黄色葡萄球菌和大肠杆菌显示出抗菌活性，且对人成骨细胞（hFob 1.19）无细胞毒性浓度，特别是当LDH结构中存在镓阳离子时。还研究了复合材料中HA的存在对骨结合能力和人成骨细胞增殖的影响。HA似乎降低了LDH对人成骨细胞的毒性，同时不影响骨结合能力。这项多学科研究提供了新型LDH和HA@LDH复合材料的生物化学、结构表征，还评估了它们的生物活性，使其有可能应用于钛基假体。
