El-Sayed Youssef A, Abdel-Moneim Ahmed E, Taha Rania G, Khalil Mona F, Abdel-Gaber Rewaida, Thagfan Felwa A, Al-Malki Esam S, Dkhil Mohamed A
Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo 11759, Egypt.
Biological and Geological Sciences Department, Faculty of Education, Ain Shams University, Roxy, Heliopolis, Cairo 11757, Egypt.
Vet Sci. 2024 Oct 10;11(10):490. doi: 10.3390/vetsci11100490.
is a parasite that causes coccidiosis in a variety of animals, leading to nutritional imbalances, issues with food digestion and absorption, low weight, and intestinal inflammation of varying degrees in its hosts. Anticoccidial medication resistance has become a significant obstacle to disease control efforts, leading to a renewed focus on discovering novel chemicals for alternative therapeutic approaches. The purpose of this study was to determine the efficacy of extract (HpE) in treating eimeriosis in mice. Five groups of mice were studied, with sporulated oocysts (1 × 10) used to infect three groups experimentally. The first group was treated with 200 mg/kg of HpE, the second group with Amprolium (120 mg/kg), and the third group was left untreated as a control. The fourth and fifth groups were uninfected, with one of them treated with 200 mg/kg of HpE and the other used as a negative control. The results revealed that HpE has 10 phytochemical compounds according to GC-MS analysis. Treatment with HpE resulted in a substantial decrease in oocyst output in feces. Also, the parasitic stages showed morphometric alterations, with reductions in the measurements compared with the infected, untreated mice. Moreover, expression of the mucin gene (MUC2) was higher in treated mice compared to infected ones, with significant increases in goblet cell numbers, which provided additional evidence for the activity of HpE as an anticoccidial product. In conclusion, there are a wide variety of natural products and many have been employed in folk medicine for treating a range of parasitic diseases.
是一种可在多种动物中引起球虫病的寄生虫，会导致宿主营养失衡、食物消化和吸收问题、体重减轻以及不同程度的肠道炎症。抗球虫药物耐药性已成为疾病控制工作的重大障碍，这使得人们重新关注寻找用于替代治疗方法的新型化学物质。本研究的目的是确定提取物（HpE）治疗小鼠艾美耳球虫病的疗效。研究了五组小鼠，用（1×10）个孢子化卵囊对三组进行实验性感染。第一组用200mg/kg的HpE治疗，第二组用氨丙啉（120mg/kg）治疗，第三组不治疗作为对照。第四组和第五组未感染，其中一组用200mg/kg的HpE治疗，另一组作为阴性对照。结果显示，根据气相色谱-质谱分析，HpE含有10种植物化学成分。用HpE治疗导致粪便中卵囊产量大幅下降。此外，寄生虫阶段显示出形态计量学改变，与未治疗的感染小鼠相比，测量值有所降低。而且，与感染小鼠相比，治疗小鼠中粘蛋白基因（MUC2）的表达更高，杯状细胞数量显著增加，这为HpE作为抗球虫产品的活性提供了额外证据。总之，有各种各样的天然产物，许多已被用于民间医学治疗一系列寄生虫病。
