Kiniger Caterina, Zorgevica-Pockevica Ligita, Vincenti Simona
Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland.
Vet Sci. 2024 Oct 15;11(10):507. doi: 10.3390/vetsci11100507.
Surgical resection of subcutaneous neoplasms with clear margins is crucial for preventing local recurrence and avoiding adjuvant treatments. However, the evaluation of surgical margins often differs significantly from the histopathological assessment due to tissue shrinkage, which can result in inaccurate therapeutic assessments and unreliable patient prognoses. In this study, ten feline cadavers were utilized. Six 50 mm diameter specimens were collected from three regions (thorax, flank, femur) and measured at three time points: T0 (excision time), T1 (10 min after incision), and T2 (at least 48 h after sample collection and formalin fixation). Samples in the study group were stretched and fixed on a cork plate with pinpoint needles after excision to restore their original dimensions. All specimens exhibited a similar trend. After 48 h of formalin fixation, the control specimens showed significant shrinkage, with a reduction of 25.73% in radius and 26.32% in diameter. In contrast, the study specimens demonstrated minimal changes, with a radius reduction of -0.28% and no change in diameter. The results indicate that all feline skin specimens experienced significant shrinkage of approximately one-quarter from their pre-incisional size. Stretching and pinning the excised tissues allowed for the restoration and maintenance of original dimensions even after formalin fixation. This technique represents a valid and practical approach to minimize tissue shrinkage.
手术切除皮下肿瘤时保证切缘清晰对于预防局部复发和避免辅助治疗至关重要。然而，由于组织收缩，手术切缘的评估往往与组织病理学评估存在显著差异，这可能导致治疗评估不准确和患者预后不可靠。在本研究中，使用了十只猫的尸体。从三个部位（胸部、胁腹、股骨）采集了六个直径50毫米的标本，并在三个时间点进行测量：T0（切除时）、T1（切开后10分钟）和T2（样本采集并福尔马林固定后至少48小时）。研究组的样本在切除后用细针拉伸并固定在软木板上，以恢复其原始尺寸。所有标本都呈现出相似的趋势。福尔马林固定48小时后，对照标本出现明显收缩，半径减小25.73%，直径减小26.32%。相比之下，研究标本变化极小，半径减小-0.28%，直径无变化。结果表明，所有猫皮肤标本从切开前的大小收缩了约四分之一。拉伸并固定切除的组织即使在福尔马林固定后也能恢复并保持原始尺寸。该技术是一种有效且实用的方法，可最大程度减少组织收缩。
