Xie Huicheng, Fu Yikang, Fu Degang, Lin Dengfeng, Zhou Huimin, Fu Guilong, Li Hui, Liu Jinxin, Zheng Xiuguo, Li Kun
College of Agriculture and Forestry Science, Linyi University, Linyi 276000, China.
College of Forestry, Shandong Agricultural University, Tai'an 271018, China.
Toxics. 2024 Oct 20;12(10):763. doi: 10.3390/toxics12100763.
In this study, the effects of different concentrations of 2,4-dinitrophenol (2,4-DNP) stress on physiological parameters, as well as the uptake and removal of 2,4-DNP in , were investigated using hydroponic simulation experiments to explore the potential of the use of in the phytoremediation of wastewater polluted by 2,4-DNP. The results showed that (net photosynthetic rate), (transpiration rate), (stomatal conductance), (stomatal limitation value), / (maximal quantum yield of PSII photochemistry), and (photochemical quenching coefficient) of seedlings showed an overall decreasing trend, while (intercellular CO concentration) showed an increasing trend with the increase in 2,4-DNP concentration. The net photosynthetic rate and intercellular carbon dioxide concentration showed an opposite trend in the leaves with the increase in 2,4-DNP stress concentration, and the inhibition of net photosynthesis by 2,4-DNP on seedlings was mainly based on non-stomatal factors. In the 15 d incubation experiment, the values of SOD (superoxide dismutase), POD (peroxidase), and CAT (catalase) indexes were higher at low concentrations of 2,4-DNP stress, and all three enzymes reached their maximum values at 10 mg L of 2,4-DNP and then decreased. seedlings could tolerate 2,4-DNP stress well, which did not exceed 20 mg L. The toxicity of 2,4-DNP solution was significantly reduced after purification by seedlings. The removal rate of 2,4-DNP was higher than 80% in each treatment group by purified after 15 days. When the concentration of 2,4-DNP reached 20 mg L, the contents of MDA (malonicdialdehyde) were 55.62 mmol g, and the values of REC (relative conductivity) and LD (leaf damage) were 63.51% and 59.93%, respectively. The structure and function of the cell membrane in leaves were seriously damaged. With the increase in 2,4-DNP concentration, the removal of 2,4-DNP by seedlings showed a decreasing trend. When the 2,4-DNP concentration was 5 mg L, the highest removal rate of 2,4-DNP by seedlings was 95.98%, while when the 2,4-DNP concentration was 20 mg L, the highest removal rate was 86.76%. It is noted that the suitable, recommended concentration for the phytoremediation of 2,4-DNP contamination by seedlings is between 8.81 and 13.78 mg L.
本研究采用水培模拟实验，研究了不同浓度的2,4 -二硝基苯酚（2,4 - DNP）胁迫对生理参数的影响，以及[植物名称]对2,4 - DNP的吸收和去除情况，以探讨[植物名称]在植物修复受2,4 - DNP污染废水方面的应用潜力。结果表明，随着2,4 - DNP浓度的增加，[植物名称]幼苗的净光合速率（Pn）、蒸腾速率（Tr）、气孔导度（Gs）、气孔限制值（Ls）、PSII光化学最大量子产量（Fv/Fm）和光化学猝灭系数（qP）总体呈下降趋势，而胞间CO₂浓度（Ci）呈上升趋势。随着2,4 - DNP胁迫浓度的增加，叶片中净光合速率和胞间二氧化碳浓度呈现相反趋势，2,4 - DNP对[植物名称]幼苗净光合作用的抑制主要基于非气孔因素。在15 d的培养实验中，低浓度2,4 - DNP胁迫下超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）指标值较高，三种酶在2,4 - DNP浓度为10 mg/L时均达到最大值，随后下降。[植物名称]幼苗能较好地耐受2,4 - DNP胁迫，其耐受浓度不超过20 mg/L。经[植物名称]幼苗净化后，2,4 - DNP溶液的毒性显著降低。15 d后，各处理组经[植物名称]净化的2,4 - DNP去除率均高于80%。当2,4 - DNP浓度达到20 mg/L时，丙二醛（MDA）含量为55.62 μmol/g，相对电导率（REC）和叶片损伤度（LD）分别为63.51%和59.93%，叶片细胞膜的结构和功能受到严重破坏。随着2,4 - DNP浓度的增加，[植物名称]幼苗对2,4 - DNP的去除率呈下降趋势。当2,4 - DNP浓度为5 mg/L时，[植物名称]幼苗对2,4 - DNP的最高去除率为95.98%，而当2,4 - DNP浓度为20 mg/L时，最高去除率为86.76%。值得注意的是，[植物名称]幼苗修复2,4 - DNP污染的适宜推荐浓度在8.81至13.78 mg/L之间。
