Department of Soil Sciences, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran.
J Environ Manage. 2020 Feb 15;256:109740. doi: 10.1016/j.jenvman.2019.109740. Epub 2019 Dec 14.
Among hazardous pollutants, 2,4-Dinitrophenol (2,4-DNP) is considered highly toxic and possesses a remarkable resistance to degradation. Therefore, investigation of the possible mechanisms for removal of such pollutants is important. Laccase enzyme can decompose phenolics despite the fact that its application has been limited due to lack of possibility to reuse it. Immobilization can overcome this problem. In this paper, laccase complexes with montmorillonite K10 and zeolite were used to decompose 2,4-DNP with concentrations of 1.5 mg l and 50 mg kg in synthetic wastewater and soil, respectively. The maximum removal of pollutant from wastewater in samples containing laccase-zeolite and laccase-montmorillonite complexes were 99 and 93.3%, respectively, which occurred at 4 h incubation compared with 6 h for free laccase. The maximum removal of pollutant from soil was observed for all treatments after 16 h of incubation. The maximum removal for samples containing free laccase, laccase-zeolite, and laccase-montmorillonite complexes were 98.5%, 98.6%, and 90.4%, respectively. Control sample also showed maximum removal of 35.8%. In general, application of laccase-zeolite complexes in aqueous environment, and these complexes and free laccases in soil was found very effective in degradation of 2,4-DNP. Hence, the use of laccase, especially immobilized laccases, for removal of 2,4-DNP from environment is promising.
在危险污染物中，2,4-二硝基苯酚（2,4-DNP）被认为是剧毒物质，具有很强的抗降解能力。因此，研究去除这种污染物的可能机制非常重要。漆酶可以分解酚类物质，尽管由于其难以重复使用，其应用受到限制。固定化可以克服这个问题。在本文中，使用漆酶与蒙脱石 K10 和沸石的复合物来分解浓度分别为 1.5mg/L 和 50mg/kg 的合成废水中的 2,4-DNP 和土壤中的 2,4-DNP。在含有漆酶-沸石和漆酶-蒙脱石复合物的样品中，污染物从废水中的最大去除率分别为 99%和 93.3%，与游离漆酶的 6 小时相比，在 4 小时孵育时达到。所有处理的土壤中污染物的最大去除率在 16 小时孵育后观察到。含有游离漆酶、漆酶-沸石和漆酶-蒙脱石复合物的样品的最大去除率分别为 98.5%、98.6%和 90.4%。对照样品的最大去除率也达到了 35.8%。总的来说，在水相环境中应用漆酶-沸石复合物，以及在土壤中应用这些复合物和游离漆酶，对于 2,4-DNP 的降解非常有效。因此，使用漆酶，特别是固定化漆酶，从环境中去除 2,4-DNP 是有前途的。
