Rossant J, Vijh K M, Grossi C E, Cooper M D
Nature. 1986;319(6053):507-11. doi: 10.1038/319507a0.
The liver of the neonatal mouse continues to show haematopoietic activity for up to 2 weeks after birth and morphological analysis has shown that this activity becomes focused in discrete haematopoietic colonies by the end of the first week postnatal. Furthermore, each colony contains cells of one haematopoietic lineage only, that is, erythroid, myeloid or pre-B-lymphoid cells. This pattern of differentiation suggests that each colony is derived from a single committed precursor cell, which, if true, would represent the first demonstration of non-mixed haematopoietic colonies in normal development and would provide a useful system for studying the factors affecting the clonal diversity of haematopoietic stem cells and their lineage-committed progeny. Here we have analysed the haematopoietic foci in the liver of neonatal mouse chimaeras, using a newly developed ubiquitous in situ cell marker system which clearly demonstrates the clonal origin of these colonies.
新生小鼠的肝脏在出生后长达2周的时间内持续表现出造血活性，形态学分析表明，在出生后第一周结束时，这种活性集中在离散的造血集落中。此外，每个集落仅包含一种造血谱系的细胞，即红细胞系、髓细胞系或前B淋巴细胞系细胞。这种分化模式表明每个集落都源自单个定向祖细胞，如果真是这样，这将是正常发育中非混合造血集落的首次证明，并将为研究影响造血干细胞及其定向祖细胞克隆多样性的因素提供一个有用的系统。在这里，我们使用一种新开发的普遍存在的原位细胞标记系统，分析了新生小鼠嵌合体肝脏中的造血灶，该系统清楚地证明了这些集落的克隆起源。
