Houssaint E, Hallet M M
Centre de Recherche de Biologie et de Physico-Chimie Cellulaires, Faculté des Sciences, Nantes, France.
Immunology. 1988 Jul;64(3):463-7.
Liver rudiments were removed from mouse embryos at Day 9 of gestation, before the 28-somite stage, when they are still not colonized by extrinsic haemopoietic stem cells (HSC), and they were grafted under the kidney capsule of syngeneic adult mice. Hepatocytes differentiated normally, but no colonization by HSC occurred. As control, we used precolonized thymic rudiments taken at Day 10 of gestation, when they are still included in the third branchial arches. They were grafted in the same conditions as liver rudiments and they became colonized by extrinsic HSC that gave rise to lymphocytes. So, adult HSC are not able to colonize the fetal liver rudiment while they have the capacity to home the fetal thymus. We discuss these results in terms of an ontogenic maturation of HSC that could change their homing capacities around birth.
在妊娠第9天，即28体节期之前，当肝脏原基尚未被外源性造血干细胞（HSC）定植时，从小鼠胚胎中取出肝脏原基，并将其移植到同基因成年小鼠的肾包膜下。肝细胞正常分化，但未发生HSC的定植。作为对照，我们使用在妊娠第10天取出的预先定植的胸腺原基，此时它们仍包含在第三鳃弓中。将它们在与肝脏原基相同的条件下进行移植，它们被外源性HSC定植，这些HSC产生淋巴细胞。因此，成年HSC虽然有能力归巢到胎儿胸腺，但却无法定植到胎儿肝脏原基。我们根据HSC的个体发育成熟来讨论这些结果，这种成熟可能会在出生前后改变它们的归巢能力。
