Su Xin-Yi, Li Shu, Fan Ying-Ying, Wei Tai, Lu Zhi-Yue, Ye Peng
Department of Stomatology, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences.
First Clinical Division, Peking University School and Hospital of Stomatology, Beijing, China.
J Craniofac Surg. 2024 Oct 25;36(2):e130-6. doi: 10.1097/SCS.0000000000010807.
This experimental study aimed to assess the three-dimensional and histological changes of the alveolar socket with local application of ZA.
Nine male New Zealand white rabbits were randomly allocated into 4-, 8-, and 12-week groups after the extraction of 4 incisor teeth. The upper and lower right sockets were filled with ZA + collagen sponge (ZA-CS group), while the left sockets were filled with collagen sponge alone (CS group) postoperatively. At 4, 8, and 12 weeks (T1, T2, and T3) after the extraction, radiographic and histomorphometric evaluations were conducted for both groups.
The increase in alveolar bone density of the ZA-CS group at T2 and T3 was significantly higher than the CS group (P＜0.01). Three-dimensional evaluations demonstrated no statistically significant differences in ridge height and width between the 2 groups at T2 and T3. On histological evaluation, the ZA-CS group included significantly fewer TRAP-positive cells than the CS group at T1 (P＜0.05). In ALP staining, all cases in both groups were classified as positive at each time point. Masson trichrome staining showed significantly higher mean red collagen volume fraction in the ZA-CS group (76.09%, 79.64%) than in the CS group (66.17%, 69.22%) at T2 and T3, respectively (P＜0.05 and ＜0.01).
Although local ZA application with collagen sponge did not reduce alveolar ridge contraction, it improved the bone density and maturity of newly formed bone after tooth extraction.
本实验研究旨在评估局部应用唑来膦酸（ZA）后牙槽窝的三维及组织学变化。
9只雄性新西兰白兔拔除4颗切牙后随机分为4周、8周和12周组。术后，右上颌和右下颌牙槽窝填充ZA+胶原海绵（ZA-CS组），而左牙槽窝仅填充胶原海绵（CS组）。拔牙后4周、8周和12周（T1、T2和T3），对两组进行影像学和组织形态计量学评估。
ZA-CS组在T2和T3时牙槽骨密度的增加显著高于CS组（P＜0.01）。三维评估显示，两组在T2和T3时牙槽嵴高度和宽度无统计学显著差异。组织学评估显示，ZA-CS组在T1时抗酒石酸酸性磷酸酶（TRAP）阳性细胞显著少于CS组（P＜0.05）。在碱性磷酸酶（ALP）染色中，两组所有病例在各时间点均分类为阳性。Masson三色染色显示，ZA-CS组在T2和T3时平均红色胶原体积分数分别显著高于CS组（76.09%，79.64%比66.17%，69.22%）（P＜0.05和＜0.01）。
虽然局部应用ZA联合胶原海绵并未减少牙槽嵴收缩，但它改善了拔牙后新形成骨的骨密度和成熟度。
