The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, The Key Laboratory of Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
Department of Hematology, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen 361005, China.
Anal Chem. 2024 Nov 5;96(44):17897-17906. doi: 10.1021/acs.analchem.4c04769. Epub 2024 Oct 25.
Accurate identification of tumor-derived exosomes is crucial for advancing cancer diagnosis and therapies. However, distinguishing tumor-derived exosomes is challenging due to the heterogeneity of exosomes, which reflect different sizes and cells of origin. To address this challenge, we introduce the urvature and ntigen-mediated roximity ligation assay for mo-derived xosomes () strategy, which leverages the size-selective properties of curvature-sensing peptides and specific antigen binding of aptamers. CAPTURE enables highly specific identification and precise quantification of the PD-L1 exosomes in plasma samples. CAPTURE is proven to be simple, homogeneous, rapid, and highly selective, achieving a 100% specificity in discriminating colorectal cancer (CRC) patients from healthy donors. Overall, the CAPTURE strategy presents a promising avenue for precise and noninvasive cancer diagnosis.
准确识别肿瘤来源的外泌体对于推进癌症诊断和治疗至关重要。然而，由于外泌体的异质性，区分肿瘤来源的外泌体具有挑战性，因为外泌体反映了不同的大小和起源细胞。为了解决这一挑战，我们引入了基于曲率和抗原介导的邻近连接分析用于肿瘤衍生的外泌体（）策略，该策略利用了曲率感应肽的尺寸选择性和适体的特异性抗原结合。捕获能够高度特异性地识别和精确定量血浆样本中的 PD-L1 外泌体。已证明捕获方法简单、均相、快速且高度选择性，在区分结直肠癌（CRC）患者和健康供体方面实现了 100%的特异性。总体而言，捕获策略为精确和非侵入性的癌症诊断提供了一个有前途的途径。
