Key Laboratory of Animal pathogen Infection and Immunology of Fujian Province, College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Joint Laboratory of Animal Pathogen Prevention and Control of Fujian-Nepal, College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Key Laboratory of Animal pathogen Infection and Immunology of Fujian Province, College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Joint Laboratory of Animal Pathogen Prevention and Control of Fujian-Nepal, College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Vet Microbiol. 2024 Nov;298:110277. doi: 10.1016/j.vetmic.2024.110277. Epub 2024 Oct 18.
Vaccine adjuvants, serving as non-specific immune enhancers, play a pivotal role in the immunoprevention and control of animal diseases. This study utilized prokaryotic expression systems to express and purify chicken-derived cIFITM1, cIFITM3, and cViperin, which were then formulated as adjuvants with H9N2 avian influenza virus antigens to create inactivated vaccines. These vaccines were administered to SPF chickens to investigate their immunopotentiating functions. Additionally, the proteins were assessed for their ability to act as standalone immune enhancers. The results demonstrated that cIFITM1, cIFITM3, and cViperin significantly elevated serum hemagglutination inhibition (HI) antibody titers. Notably, when used individually, these proteins markedly enhanced the antiviral capabilities of challenged chickens, leading to alleviated clinical symptoms, reduced tracheal virus replication, diminished virus shedding, and lessened histopathological damage, with cIFITM1 exhibiting the most pronounced effect. Furthermore, the protective efficacy of two H9N2 recombinant virus inactivated vaccines supplemented with cIFITM1 adjuvant was validated, achieving a 100 % vaccine protection efficiency. In conclusion, cIFITM1, cIFITM3, and cViperin as adjuvants for influenza vaccines effectively inhibit virus replication and shedding, highlighting their significant potential in influenza prevention and control.
疫苗佐剂作为非特异性免疫增强剂，在动物疾病的免疫预防和控制中发挥着关键作用。本研究利用原核表达系统表达和纯化鸡源 cIFITM1、cIFITM3 和 cViperin，并将其与 H9N2 禽流感病毒抗原联合作为佐剂制备灭活疫苗。将这些疫苗接种 SPF 鸡，以研究其免疫增强功能。此外，还评估了这些蛋白质作为独立免疫增强剂的作用。结果表明，cIFITM1、cIFITM3 和 cViperin 显著提高了血清血凝抑制（HI）抗体滴度。值得注意的是，当单独使用时，这些蛋白质显著增强了受挑战鸡的抗病毒能力，导致临床症状减轻，气管病毒复制减少，病毒脱落减少，组织病理学损伤减轻，其中 cIFITM1 效果最为显著。此外，还验证了两种补充 cIFITM1 佐剂的 H9N2 重组病毒灭活疫苗的保护效力，达到了 100%的疫苗保护效率。综上所述，cIFITM1、cIFITM3 和 cViperin 作为流感疫苗佐剂可有效抑制病毒复制和脱落，为流感的预防和控制提供了重要的潜在价值。
