小麦醇溶蛋白衍生肽对萎缩性乳糜泻肠黏膜腺苷酸环化酶的体外激活作用。

In vitro activation of adenylate cyclase of atrophic celiac intestinal mucosa by wheat gliadin-derived peptides.

作者信息

Beguinot L, Auricchio S, Bernardin J E, de Ritis G, De Vincenzi M, Kasarda D D, Macchia V, Silano V

出版信息

Pediatr Res. 1986 Jan;20(1):42-4. doi: 10.1203/00006450-198601000-00011.

DOI:10.1203/00006450-198601000-00011

Abstract

In order to demonstrate that gliadin peptides may interact with cell membranes of celiac small intestinal mucosa, the capacity of these peptides to activate the cell membrane enzyme adenylate cyclase was tested. The addition of peptides from bread wheat purified A-gliadin and whole gliadin (proteins that are toxic for celiac patients) enhanced the adenylate cyclase activity of crude cell membrane preparations obtained from atrophic small intestinal mucosa of celiac patients. No activation of adenylate cyclase of this tissue was observed with peptides from proteins nontoxic for celiac patients (bread wheat albumin and maize prolamin). Gliadin peptides did not activate adenylate cyclase of morphologically normal small intestinal mucosa from normal subjects or from celiac patients in remission. These results, therefore, suggest that peptides from bread wheat gliadin may interact with cell membrane of atrophic small intestinal mucosa of celiac patients.

摘要

为了证明麦醇溶蛋白肽可能与乳糜泻患者小肠黏膜的细胞膜相互作用，测试了这些肽激活细胞膜酶腺苷酸环化酶的能力。添加从小麦纯化的A-麦醇溶蛋白和全麦醇溶蛋白（对乳糜泻患者有毒的蛋白质）中的肽，可增强从乳糜泻患者萎缩性小肠黏膜获得的粗制细胞膜制剂的腺苷酸环化酶活性。对于对乳糜泻患者无毒的蛋白质（小麦白蛋白和玉米醇溶蛋白）中的肽，未观察到该组织的腺苷酸环化酶被激活。麦醇溶蛋白肽不会激活正常受试者或处于缓解期的乳糜泻患者形态正常的小肠黏膜的腺苷酸环化酶。因此，这些结果表明，小麦麦醇溶蛋白中的肽可能与乳糜泻患者萎缩性小肠黏膜的细胞膜相互作用。