Peracchi M, Bamonti-Catena F, Faggioli P, Molteni N, Bardella M T, Bareggi B, Bianchi P A
Istituto di Scienze Mediche, Università di Milano, IRCCS Ospedale Policlinico, Milan, Italy.
Gut. 1993 Jun;34(6):769-73. doi: 10.1136/gut.34.6.769.
This study measured the values of cyclic nucleotides and adenylate and guanylate cyclase activities in duodenal mucosa homogenates to verify if they played a part in coeliac disease. Nine controls, 13 patients who did not receive treatment and nine patients who received treatment were studied. Cyclase activity assays were performed under basal conditions and in the presence of gliadin derived peptides. Duodenal mucosa cyclic nucleotide values and adenylate cyclase activity were significantly higher in patients who did not receive treatment than in those who did and in controls, whereas guanylate cyclase activity was similar in all groups. Gliadin derived peptides did not affect guanylate cyclase activity, but significantly increased adenylate cyclase activity in homogenates from patients who did not receive treatment. As extracellular cyclic nucleotide concentrations could reflect changes in their intracellular metabolism, plasma and urine cyclic nucleotide values were also measured in 25 controls and in 55 patients studied at different stages of their disease. Extracellular cyclic nucleotides were considerably high in patients who were not healed and became normal after about one year of treatment. These data suggest that cyclic nucleotides may participate in the pathophysiological processes of coeliac disease.
本研究测定了十二指肠黏膜匀浆中环核苷酸、腺苷酸和鸟苷酸环化酶活性的值，以验证它们是否在乳糜泻中起作用。研究了9名对照者、13名未接受治疗的患者和9名接受治疗的患者。在基础条件下以及存在麦醇溶蛋白衍生肽的情况下进行环化酶活性测定。未接受治疗的患者十二指肠黏膜环核苷酸值和腺苷酸环化酶活性显著高于接受治疗的患者和对照者，而鸟苷酸环化酶活性在所有组中相似。麦醇溶蛋白衍生肽不影响鸟苷酸环化酶活性，但显著增加未接受治疗患者匀浆中的腺苷酸环化酶活性。由于细胞外环核苷酸浓度可反映其细胞内代谢的变化，还对25名对照者和55名处于疾病不同阶段的患者进行了血浆和尿液环核苷酸值的测定。未治愈患者的细胞外环核苷酸含量相当高，治疗约一年后恢复正常。这些数据表明，环核苷酸可能参与乳糜泻的病理生理过程。
