Martin M T, Jacobs F A, Brushmiller J G
Proc Soc Exp Biol Med. 1986 Feb;181(2):249-55. doi: 10.3181/00379727-181-42249.
The aim of this study was to detect and identify major low molecular weight (less than 10,000) copper-binding ligands in human bile. Modified gel chromatography was used as the method of ligand detection because it ensures the detection of labile as well as inert metal-ligand complexes. Conjugated bilirubin, peptides, and amino acids, primarily glycine, were isolated as the major ligands. In contrast to the other copper-binding ligands, the peptides were poor zinc binders, suggesting the possibility that they may confer necessary specificity to trace metal elimination.
本研究的目的是检测和鉴定人胆汁中主要的低分子量（小于10,000）铜结合配体。采用改良的凝胶色谱法作为配体检测方法，因为它能确保检测不稳定以及惰性的金属-配体复合物。共轭胆红素、肽和氨基酸（主要是甘氨酸）被分离为主要配体。与其他铜结合配体不同，这些肽是较差的锌结合剂，这表明它们可能赋予痕量金属消除必要特异性的可能性。
