McCullars G M, O'Reilly S, Brennan M
Clin Chim Acta. 1977 Jan 3;74(1):33-8. doi: 10.1016/0009-8981(77)90384-9.
The copper profile of human bile was studied using a Cu-free preparative polyacrylamide gel system. 85 percent and 91 percent of the Cu in the bile of two subjects was found in a leading pigmented band with no additional detectable copper in the remainder of the gel in either subject. The content of the pigmented band was recovered from the gel by an intermediate electrophoretic technique. Subsequent n-butanol extraction removed contaminating bile salts with the formation of a Cu containing pigmented precipitate. Using thin-layer cellulose chromatography this precipitate was separated into two pigments which on the basis of color and diazotization reaction were believed to be conjugated bilirubins. Addition of Cu transformed the pigments into biochemically different species. 64Cu verified copper binding by the altered pigments.
使用无铜制备聚丙烯酰胺凝胶系统研究了人胆汁中的铜分布情况。在两名受试者的胆汁中，85%和91%的铜存在于一条主要的有色带中，在这两名受试者的凝胶其余部分均未检测到额外的铜。通过中间电泳技术从凝胶中回收有色带的成分。随后的正丁醇萃取去除了污染的胆汁盐，形成了含铜的有色沉淀。使用薄层纤维素色谱法将该沉淀分离成两种色素，根据颜色和重氮化反应，认为它们是结合胆红素。铜的添加将这些色素转化为生物化学性质不同的物质。64Cu证实了改变后的色素与铜的结合。
