Cao Xiaonian, Hou Yunfei, Liu Qingqing, Yang Qian, Liu Min, Lin Haixu, Ren Qingxi, Mao Jian
Luzhou Laojiao Co., Ltd., Luzhou 646000, China.
School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Foods. 2024 Oct 18;13(20):3316. doi: 10.3390/foods13203316.
The unique flavour contribution of higher alcohols in alcoholic beverages has received growing attention; however, there is a dearth of information on their in vivo metabolic kinetics. In this study, the composition and content of higher alcohols in different alcoholic beverages from Chinese and were studied via in vivo analysis using Bama pigs to elucidate the mechanisms for intoxication of alcohol in vitro and in drinkers. Direct injection combined with gas chromatography-mass spectrometry (GC-MS) were used to accurately quantify a total of 14 higher alcohols in five alcoholic beverages. Based on the external standard method, a total content of 289.37-938.33 mg/L was detected, mainly 1-butanol, 3-methyl-1-butanol, 1-hexanol, 2-methyl-1-propanol and 2-butanol. Then, headspace solid-phase microextraction (HS-SPME) and solid-phase extraction (SPE) combined with GC-MS analysis strategy, respectively, were adopted to continuously monitor the changes in the concentrations of ethanol and 11 higher alcohols in the blood within 24 h after gavage of different alcoholic beverages, and the key pharmacokinetic parameters were analysed. The peak concentration (C) and area under curve (AUC) of blood higher alcohols were significantly lower than those of ethanol ( 0.05), accompanied by a later peak time (T) and a larger apparent clearance rate (CL_F), and there were certain differences between the same higher alcohols in different alcoholic beverages and between different higher alcohols in the same alcoholic beverage. This work provides valuable insights into the metabolism of alcoholic beverages.
高级醇类在酒精饮料中独特的风味贡献受到了越来越多的关注；然而，关于它们在体内的代谢动力学信息却十分匮乏。在本研究中，通过巴马猪体内分析，研究了中国不同酒精饮料中高级醇类的组成和含量，以阐明酒精在体外和饮酒者体内的中毒机制。采用直接进样结合气相色谱 - 质谱联用（GC - MS）技术准确测定了五种酒精饮料中总共14种高级醇类的含量。基于外标法，检测到总含量为289.37 - 938.33 mg/L，主要为正丁醇、3 - 甲基 - 1 - 丁醇、正己醇、2 - 甲基 - 1 - 丙醇和2 - 丁醇。然后，分别采用顶空固相微萃取（HS - SPME）和固相萃取（SPE）结合GC - MS分析策略，连续监测不同酒精饮料灌胃后24 h内血液中乙醇和11种高级醇类浓度的变化，并分析关键药代动力学参数。血液中高级醇类的峰浓度（C）和曲线下面积（AUC）显著低于乙醇（P < 0.05），同时峰时间（T）较晚，表观清除率（CL_F）较大，且不同酒精饮料中相同高级醇类以及同一酒精饮料中不同高级醇类之间存在一定差异。本研究为酒精饮料的代谢提供了有价值的见解。
