State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Int J Mol Sci. 2024 Oct 15;25(20):11070. doi: 10.3390/ijms252011070.
, a macrofungal species in the , , is known to enhance the nutritional and bioactive properties of rice through co-fermentation; however, its own secondary metabolites are not well understood. In this study, an integrative analysis of transcriptome and metabolome data revealed that the accumulation of steroids, steroid derivatives, and triterpenoids in peaks during the mid-growth stage, while the genes associated with these metabolites show higher expression levels from the early to mid-growth stages. Weighted gene co-expression network analysis identified several modules containing candidate genes involved in the synthesis of steroids, steroid derivatives, and triterpenoids. Specifically, six key hub genes were identified, along with their connectivity to other related genes, as potential catalysts in converting the precursor lanosterol to celastrol. This study enhances our understanding of the secondary metabolites of and is essential for the selective utilization of these bioactive compounds.
, 一种属于 的大型真菌，通过共发酵提高了大米的营养价值和生物活性；然而，其自身的次生代谢产物还不是很清楚。在这项研究中，转录组和代谢组数据的综合分析表明，在中期生长阶段， 在 中积累类固醇、类固醇衍生物和三萜，而与这些代谢物相关的基因在早期到中期生长阶段表现出更高的表达水平。加权基因共表达网络分析确定了几个包含参与类固醇、类固醇衍生物和三萜合成的候选基因的模块。具体来说，确定了六个关键的枢纽基因，以及它们与其他相关基因的连接，作为将前体羊毛甾醇转化为雷公藤红素的潜在催化剂。这项研究提高了我们对 的次生代谢产物的理解，对于选择性利用这些生物活性化合物至关重要。
