TCM and Ethnomedicine Innovation and Development International Laboratory, Innovative Material Medical Research Institute, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, China.
School of Pharmacy, Hunan University of Chinese Medicine, Changsha, China.
BMC Genomics. 2024 Jul 19;25(1):706. doi: 10.1186/s12864-024-10608-4.
According to Chinese ancient books, both fruits and rhizomes of Polygonatum cyrtonema Hua have medicinal and edible values. Up to now, there is no report about the metabolite profiles and regulatory network in fruits and different year-old rhizomes of P. cyrtonema.
In this study, we performed integrative analyses of metabolome and transcriptome to reveal the dynamic accumulation and regulatory network of fruits and different year-old rhizomes in P. cyrtonema. The relative content of phenolic acids, lignans and coumarins, flavonoids and alkaloids increased with growth years, while steroids and lipids decreased with it. In addition, the relative content of nucleotides and derivatives, flavonoids, organic acids, steroids and lipids in fruits were higher than rhizomes. Genes that might relate to the biosynthesis of polysaccharides, flavonoids, triterpene saponins and alkaloids biosynthesis were further analyzed by transcriptome analysis, including sacA, GMPP, PMM, CCoAOMT, CHI, ANR, CHS, DXS, GGPS, ZEP, CYP72A219 and so on, for their expressions were positively correlated with the relative content of the metabolites. Additionally, the correlation network in sugar and aromatic amino acids metabolites were constructed to further illustrate the biosynthesis of polysaccharides, flavonoids and alkaloids in P. cyrtonema, and some transcription factors (TFs) were screened, such as C2C2, MYB, bZIP, GRAS and NAC.
This study can deepen our understanding of the accumulation patterns and molecular mechanism of the main compounds in P. cyrtonema, and provide reference for the standardize production of P. cyrtonema.
根据中国古代书籍记载，黄精的果实和根茎都具有药用和食用价值。迄今为止，还没有关于黄精果实和不同年份根茎中代谢物谱和调控网络的报道。
在这项研究中，我们对代谢组学和转录组学进行了综合分析，以揭示黄精果实和不同年份根茎中的动态积累和调控网络。酚酸、木脂素和香豆素、类黄酮和生物碱的相对含量随着生长年份的增加而增加，而甾体和脂质则随着生长年份的增加而减少。此外，果实中核苷酸及其衍生物、类黄酮、有机酸、甾体和脂质的相对含量高于根茎。通过转录组分析进一步分析了可能与多糖、类黄酮、三萜皂苷和生物碱生物合成有关的基因，包括 sacA、GMPP、PMM、CCoAOMT、CHI、ANR、CHS、DXS、GGPS、ZEP、CYP72A219 等，因为它们的表达与代谢物的相对含量呈正相关。此外，还构建了糖和芳香族氨基酸代谢物的相关网络，以进一步说明黄精中多糖、类黄酮和生物碱的生物合成，并筛选了一些转录因子（TFs），如 C2C2、MYB、bZIP、GRAS 和 NAC。
本研究可以加深我们对黄精中主要化合物积累模式和分子机制的理解，为黄精的规范化生产提供参考。
