（TTV）脑炎患者的感染情况。

(TTV) Infection in Patients with Encephalitis.

机构信息

Department of Immunopathology of Infectious and Parasitic Diseases, Medical University of Warsaw, Pawinskiego 3c, 02-106 Warsaw, Poland.

Department of the Medical Genetics, Medical University of Warsaw, Pawinskiego 3c, 02-106 Warsaw, Poland.

出版信息

Int J Mol Sci. 2024 Oct 17;25(20):11177. doi: 10.3390/ijms252011177.

DOI:10.3390/ijms252011177

PMID:39456958

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11508335/

Abstract

virus (TTV) is a ssDNA orphan virus belonging to the family, but some recent studies suggested its possible involvement in central nervous system (CNS) pathology. We analyzed serum and cerebrospinal fluid samples (CSF) from 109 patients with encephalitis for TTV infection using serological and molecular testing, virus quantitative measurement, and next-generation sequencing-based (NGS) phylogenetic analysis. TTV noncoding region (UTR) and/or open reading frame 1 (ORF-1) sequences were detected in serum of 86 (79%) patients and in nine (8%) patients in CSF. Five of the latter patients were coinfected with various - and . Anti-TTV-IgG were detected in 80 (73.4%) sera and in two (1.8%) CSF samples, while anti-TTV-IgM were present in three (2.8%) sera and in none of the CSFs. Phylogenic analysis of CSF-derived TTV ORF-1 sequences revealed the presence of three unique variants in one patient. TTV was quantified in five CSF-serum pairs: in two patients viral loads were similar, and in three serum TTV loads were approximately one log higher. Our results suggest at least an occasional replication of TTV in CNS. However, whether TTV could be the cause of encephalitis requires further studies.

摘要

病毒（TTV）是一种 ssDNA 孤儿病毒，属于细小病毒科，但最近的一些研究表明它可能与中枢神经系统（CNS）病理学有关。我们使用血清学和分子检测、病毒定量测量以及基于下一代测序的（NGS）系统发育分析，分析了 109 例脑炎患者的血清和脑脊液（CSF）样本中 TTV 感染情况。在血清中检测到了 TTV 非编码区（UTR）和/或开放阅读框 1（ORF-1）序列，在 86 例（79%）患者的血清和 9 例（8%）患者的 CSF 中检测到了 TTV 序列。后者的 5 例患者合并感染了各种 - 和. 在 80 例（73.4%）血清和 2 例（1.8%）CSF 样本中检测到抗 TTV-IgG，而在 3 例（2.8%）血清和无任何 CSF 样本中检测到抗 TTV-IgM。对 CSF 衍生的 TTV ORF-1 序列的系统发育分析显示，在一名患者中存在三种独特的变异体。在五个 CSF-血清对中对 TTV 进行了定量：在两名患者中病毒载量相似，而在三名患者中血清 TTV 载量高约一个对数级。我们的结果表明，TTV 至少偶尔会在 CNS 中复制。然而，TTV 是否可能是脑炎的原因还需要进一步研究。