Rodrigues Barreto Inês, Monteiro Andreia, Rocha Ernesto Fernandes, Santos Catarina Reis, Fonseca Ana Mafalda
CICS-UBI-Health Sciences Research Centre, University of Beira Interior, Av. Infante D. Henrique, 6200-506 Covilhã, Portugal.
Faculty of Health Sciences, University of Beira Interior, 6200-506 Covilhã, Portugal.
Biomedicines. 2024 Sep 26;12(10):2188. doi: 10.3390/biomedicines12102188.
Patients undergoing hemodialysis (HD) often exhibit an impaired cellular immune response, which may contribute to an increased susceptibility to infections and other complications. Th1 cells, a subset of T-helper cells, play a crucial role in cellular immunity. However, the modulation of Th1 cells by HD treatment remains unclear. This study aims to investigate the levels of circulating T cells, especially Th1 cells, and the neutrophil-to-lymphocyte ratio (NLR) in HD patients. : We recruited 26 HD patients and 10 healthy volunteers. Demographical data were collected, and peripheral blood samples were analyzed. Absolute blood cell counts were determined, and T-cell populations were identified using flow cytometry. Th1 cells were defined as IFN-γ-producing CD4 T cells after in vitro activation, and NLR was calculated through the ratio between the neutrophil and lymphocyte counts measured in peripheral blood. We have observed a significant decrease in Th1 subpopulation frequency in HD patients, as well as significant correlations between immunological and demographic parameters, among which are the NLR values and the absolute values of T-cell subsets. These results seem to clarify the role of Th1 cells in modulating the immune responses of hemodialysis-treated patients, potentially considering its frequency as an indicator for CKD development.
接受血液透析（HD）的患者通常表现出细胞免疫反应受损，这可能导致其对感染和其他并发症的易感性增加。辅助性T细胞的一个亚群Th1细胞在细胞免疫中起关键作用。然而，HD治疗对Th1细胞的调节作用仍不清楚。本研究旨在调查HD患者循环T细胞，尤其是Th1细胞的水平以及中性粒细胞与淋巴细胞比值（NLR）。我们招募了26名HD患者和10名健康志愿者。收集人口统计学数据，并对外周血样本进行分析。测定绝对血细胞计数，并使用流式细胞术鉴定T细胞亚群。体外激活后，将Th1细胞定义为产生干扰素-γ的CD4 T细胞，并通过外周血中测量的中性粒细胞与淋巴细胞计数之比计算NLR。我们观察到HD患者中Th1亚群频率显著降低，以及免疫和人口统计学参数之间存在显著相关性，其中包括NLR值和T细胞亚群的绝对值。这些结果似乎阐明了Th1细胞在调节血液透析治疗患者免疫反应中的作用，可能将其频率视为慢性肾脏病发展的一个指标。
