De Angelis Francesco, D'Arcangelo Camillo, Di Lodovico Silvia, Sorrentino Edoardo, Buonvivere Matteo, D'Ercole Simonetta
Department of Medical, Oral and Biotechnological Sciences, "G. d'Annunzio" University of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy.
Department of Pharmacy, "G. d'Annunzio" University of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy.
Biomedicines. 2024 Oct 1;12(10):2235. doi: 10.3390/biomedicines12102235.
The aim of this in vitro study was to analyze and compare the ability to adhere and form biofilm on the surface of light-cured VS heat-cured dental composite resins; Three composite resins with different chemical formulations were selected: GrandioSO (GR), Venus Diamond (VD) and Enamel Plus Hri Biofunction (BF). Disk-shaped specimens were manufactured by light-curing the composite resins (light-cured subgroups) and subjecting them to a further heat-curing cycle at 80° for 10 min (heat-cured subgroups). Specimens were analyzed for planktonic CFU count (CFU/mL), sessile CFU count (CFU/mL) and for biomass quantification (OD); The planktonic CFU count was higher in all the light-cured subgroups than in the heat-cured subgroups (light-cured: GR = 7.23 × 10, VD = 2.14 × 10, BF = 4.40 × 10; heat-cured: GR = 4.89 × 10, VD = 4.95 × 10, BF = 2.80 × 10), with a statistically significant increase for BF and VD. Focusing on the sessile CFUs, both GR (light-cured = 7.49 × 10; heat-cured = 3.97 × 10) and VD (light-cured = 2.93 × 10; heat-cured = 6.07 × 10) showed a significantly increased number of colonies in the light-cured subgroups. The OD values recorded for the light-cured BF subgroup (0.4280) were significantly increased compared to the heat-cured BF subgroup (0.1931); A more complete polymerization protocol seems to lead to a potential reduction in the risk of secondary caries.
本体外研究的目的是分析和比较光固化与热固化牙科复合树脂在表面上的黏附能力和形成生物膜的能力；选择了三种具有不同化学配方的复合树脂：GrandioSO（GR）、Venus Diamond（VD）和Enamel Plus Hri Biofunction（BF）。通过光固化复合树脂制备圆盘状标本（光固化亚组），并在80°下进行10分钟的进一步热固化循环（热固化亚组）。对标本进行浮游菌落形成单位计数（CFU/mL）、固着菌落形成单位计数（CFU/mL）和生物量定量（OD）分析；所有光固化亚组中的浮游菌落形成单位计数均高于热固化亚组（光固化：GR = 7.23×10，VD = 2.14×10，BF = 4.40×10；热固化：GR = 4.89×10，VD = 4.95×10，BF = 2.80×10），BF和VD有统计学上的显著增加。关注固着菌落形成单位，GR（光固化 = 7.49×10；热固化 = 3.97×10）和VD（光固化 = 2.93×10；热固化 = 6.07×10）在光固化亚组中的菌落数量均显著增加。光固化BF亚组记录的OD值（0.4280）与热固化BF亚组（0.1931）相比显著增加；更完整的聚合方案似乎会导致继发龋风险的潜在降低。
