Influence of Polymerization Protocol on Adhesion and Proliferation of on Three Dental Composite Resins.

The aim of this in vitro study was to analyze and compare the ability to adhere and form biofilm on the surface of light-cured VS heat-cured dental composite resins; Three composite resins with different chemical formulations were selected: GrandioSO (GR), Venus Diamond (VD) and Enamel Plus Hri Biofunction (BF). Disk-shaped specimens were manufactured by light-curing the composite resins (light-cured subgroups) and subjecting them to a further heat-curing cycle at 80° for 10 min (heat-cured subgroups). Specimens were analyzed for planktonic CFU count (CFU/mL), sessile CFU count (CFU/mL) and for biomass quantification (OD); The planktonic CFU count was higher in all the light-cured subgroups than in the heat-cured subgroups (light-cured: GR = 7.23 × 10, VD = 2.14 × 10, BF = 4.40 × 10; heat-cured: GR = 4.89 × 10, VD = 4.95 × 10, BF = 2.80 × 10), with a statistically significant increase for BF and VD. Focusing on the sessile CFUs, both GR (light-cured = 7.49 × 10; heat-cured = 3.97 × 10) and VD (light-cured = 2.93 × 10; heat-cured = 6.07 × 10) showed a significantly increased number of colonies in the light-cured subgroups. The OD values recorded for the light-cured BF subgroup (0.4280) were significantly increased compared to the heat-cured BF subgroup (0.1931); A more complete polymerization protocol seems to lead to a potential reduction in the risk of secondary caries.