Department of Medical, Oral and Biotechnological Science, University "G. d'Annunzio" Chieti -Pescara, Via dei Vestini 31, 66013 Chieti, Italy.
Department of Clinical Sciences, Polytechnic University of Marche, Via Brecce Bianche, 60100 Ancona, Italy.
Int J Mol Sci. 2020 Nov 5;21(21):8315. doi: 10.3390/ijms21218315.
This work aimed to compare the capability of to adhere to a novel surface, double-etched titanium (DAE), in respect to machined and single-etched titanium. The secondary outcome was to establish which topographical features could affect the interaction between the implant surface and bacteria. The samples' superficial features were characterized using scanning electron microscopy (SEM) and energy dispersive x-ray spectrometry (EDS), and the wetting properties were tested through sessile methods. The novel surface, the double-etched titanium (DAE), was also analyzed with atomic force microscopy (AFM). was inoculated on discs previously incubated in saliva, and then the colony-forming units (CFUs), biomass, and cellular viability were measured at 24 and 48h. SEM observation showed that DAE was characterized by higher porosity and Oxygen (%) in the superficial layer and the measurement of the wetting properties showed higher hydrophilicity. AFM confirmed the presence of a higher superficial nano-roughness. Microbiological analysis showed that DAE discs, coated by pellicle's proteins, were characterized by significantly lower CFUs at 24 and 48 h with respect to the other two groups. In particular, a significant inverse relationship was shown between the CFUs at 48 h and the values of the wetted area and a direct correlation with the water contact angle. The biomass at 24 h was slightly lower on DAE, but results were not significant concerning the other groups, both at 24 and 48 h. The DAE treatment not only modifies the superficial topography and increased hydrophilicity, but it also increases the Oxygen percentage in the superficial layer, which could contribute to the inhibition of adhesion. DAE can be considered a promising treatment for titanium implants to counteract a colonization pioneer microorganism, such as
本研究旨在比较 对新型表面（双重酸蚀钛，DAE）的黏附能力，以及对机加工和单蚀刻钛的黏附能力。次要结果是确定哪些形貌特征会影响种植体表面与细菌之间的相互作用。使用扫描电子显微镜（SEM）和能量色散 X 射线光谱（EDS）对样本的表面特征进行了表征，并通过静滴法测试了润湿性能。新型表面，双重酸蚀钛（DAE），也通过原子力显微镜（AFM）进行了分析。 将 接种到先前在唾液中孵育的圆盘上，然后在 24 和 48 小时测量菌落形成单位（CFU）、生物量和细胞活力。SEM 观察表明，DAE 的表面层具有更高的孔隙率和氧（%），润湿性测量结果表明其具有更高的亲水性。AFM 证实了表面纳米粗糙度更高的存在。微生物分析表明，与其他两组相比，DAE 圆盘在 24 和 48 小时时涂覆有牙菌斑蛋白，其 CFU 明显较低。特别是，在 48 小时时的 CFU 值与润湿面积的值呈显著负相关，而与水接触角呈正相关。在 24 小时时，DAE 上的生物量略低，但与其他两组相比，在 24 和 48 小时时均无统计学意义。DAE 处理不仅改变了表面形貌并提高了亲水性，而且还增加了表面层中的氧含量，这可能有助于抑制 的黏附。DAE 可被视为钛种植体的一种有前途的处理方法，可用于抑制类似 的定植先驱微生物。
