Zhu Xi-Rong, Jin Ye, Zhang Xue, Liu Qiu-Ning, Tang Bo-Ping
Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-Agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Wetlands, Yancheng Teachers University, Yancheng 224007, China.
College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China.
Animals (Basel). 2024 Oct 16;14(20):2981. doi: 10.3390/ani14202981.
As a key species in freshwater aquaculture, was subjected to ammonia stress to assess its impact on the hepatopancreas. A total of 4007 differentially expressed genes (DEGs) were identified between control and treatment groups, comprising 1838 upregulated and 2169 downregulated genes. Following exposure to 300 mg/L of ammonia, the oxidative phosphorylation pathway was activated, while the lysosomal pathway was suppressed, thereby influencing immune functions. Thirteen DEGs from these pathways were further validated via qRT-PCR, revealing gene expression changes of one- to two-fold. Both acid phosphatase (ACP) and alkaline phosphatase (AKP) levels in the hepatopancreas and hemolymph initially increased and then decreased, indicating a disruption in immune functionality. Additionally, alanine transaminase (ALT) and triglyceride (TG) levels were measured, alongside catalase (CAT) activity, total antioxidant capacity (T-AOC), and malondialdehyde (MDA) content, all of which showed an upward trend, signifying oxidative stress and tissue damage. These results offer critical insights into the antioxidant and immune mechanisms of in ammonia-enriched environments.
作为淡水养殖中的关键物种，[物种名称未给出]受到氨胁迫以评估其对肝胰腺的影响。在对照组和处理组之间共鉴定出4007个差异表达基因（DEG），其中包括1838个上调基因和2169个下调基因。暴露于300 mg/L的氨后，氧化磷酸化途径被激活，而溶酶体途径受到抑制，从而影响免疫功能。通过qRT-PCR进一步验证了这些途径中的13个DEG，揭示了基因表达变化为1至2倍。肝胰腺和血淋巴中的酸性磷酸酶（ACP）和碱性磷酸酶（AKP）水平最初升高然后下降，表明免疫功能受到破坏。此外，还测量了丙氨酸转氨酶（ALT）和甘油三酯（TG）水平，以及过氧化氢酶（CAT）活性、总抗氧化能力（T-AOC）和丙二醛（MDA）含量，所有这些均呈上升趋势，表明存在氧化应激和组织损伤。这些结果为[物种名称未给出]在富氨环境中的抗氧化和免疫机制提供了关键见解。
